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Anti-Herpes simplex Virus 1/2 Antibody (HRP)

Goat Anti-Viral Polyclonal Antibody

  • FC - Anti-Herpes simplex Virus 1/2 Antibody (HRP) ABD10569
    Published customer image:HIV tat/gp120-disrupted epithelial junctions facilitate HSV-1 cell-to-cell spread through junctional areas of polarized oral epithelial cells. A. Polarized tonsil cells were treated with active or inactive tat/gp120 and HIV virions for 5 days. Disrupted cells were infected with HSV-1 at 0.01 PFU per cell from basolateral membranes of polarized cells. After 3 days, cells were fixed and immunostained using goat anti-HSV immune serum (green). Cell nuclei are stained in red. Yellow represents colocalization of HSV proteins and nuclei. B. (upper panel) Plaque numbers were counted from 3 independent filter inserts and data are presented as the average number of HSV-infected plaques per insert. (lower panel) Cell-to-cell spread of HSV-1 was quantitatively evaluated by counting HSV-infected cells in the plaques. Results are presented as the average number of HSV-infected cells per plaque. Error bars indicate SEM. C. Polarized cells were infected with HSV-1. After 4 h, antibodies to nectin-1 and gD were added separately and in combination. Cell medium was changed daily to add fresh antibodies. Cells were fixed and immunostained for HSV-1, and the plaque numbers (upper panel) and the number of HSV-1-positive cells in plaques were counted (lower panel). Error bars indicate SEM. *P
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Reactivity Virus
Host Goat
Clonality Polyclonal
Isotype Polyclonal IgG
Additional Information
Immunogen Human HSV type 1, Strain F
Shelf Life 18 months from the date of despatch.
Target/Specificity Goat anti-Herpes simplex Virus 1/2 antibody reacts with Herpes Simplex virus 1 and 2 and recognizes the ICPs and late structural (virion) antigens. Goat anti-Herpes simplex Virus 1/2 antibody does not react with HEp-2 cells.Herpes Simplex virus (HSV) is a double stranded DNA virus of which there are 2 types, HSV1 and HSV2. HSV1 usually establishes latency in the trigeminal ganglion, a collection of nerve cells near the ear. From the trigeminal ganglion, it tends to recur on the lower lip or face causing cold sores. HSV2 usually resides in the sacral ganglion at the base of the spine. From there, it recurs in the genital area.
Preservative & Stabilisers 0.09% Sodium Azide (NaN3)
Storage Store at +4℃ or at -20 ℃.
PrecautionsAnti-Herpes simplex Virus 1/2 Antibody (HRP) is for research use only and not for use in diagnostic or therapeutic procedures.
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1. Sufiawati, I. and Tugizov, S.M. (2014) HIV-associated disruption of tight and adherens junctions of oral epithelial cells facilitates HSV-1 infection and spread.
PLoS One. 9: e88803. 2. Civitelli, L. et al. (2015) Herpes simplex virus type 1 infection in neurons leads to production and nuclear localization of APP intracellular domain (AICD): implications for Alzheimer's disease pathogenesis.
J Neurovirol. Apr 30. [Epub ahead of print]

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Cat# ABD10569
(40 western blots)
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