|Application ||WB, IHC-P, IHC-F, IF, E, IP|
|Purification||Purified IgG prepared by affinity chromatography on Protein G|
|Immunogen||Nuclei from myeloid leukamia biopsy cells.|
|Shelf Life||18 months from date of despatch.|
|Target/Specificity||Mouse anti-Human Histone H1 antibody, clone AE-4 recognizes histone H1.The histone proteins serve as a structural scaffold for the organization of nuclear DNA into chromatin. In eukaryotic cells there are 4 core histone proteins, H2A, H2B, H3 and H4 that assemble to form an octamer round which the DNA is wrapped in a sequence dependant manner. The resulting complex is known as a nucleosome and is the basic subunit of chromatin. Histone H1 acts as a linker and binds to each nucleosome, and by self affinity, links these nucleosomes together resulting in the 30 nm chromatin fiber.In blots of rat brain homogenate a duplet is observed at 27-29 kDa consistent with Histone H1. Speckled staining is observed in the nucleus of normal and malignant cells.|
|Preservative & Stabilisers||0.09% Sodium Azide (NaN3)|
|Storage||Store at +4℃ or at -20 ℃.|
|Precautions||Anti-Human Histone H1 Antibody, clone AE-4 is for research use only and not for use in diagnostic or therapeutic procedures.|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
1. Swindle, C. S. & Engler, J. A. (1998) Association of the human papillomavirus type 11 E1 protein with histone H1.J. Virol. 72(3): 1994-2001. 2. Hole, P.S. et al. (2010) Ras-induced reactive oxygen species promote growth factor-independentproliferation in human CD34+ hematopoietic progenitor cells.Blood. 115:1238-46.2. Wang, X. et al. (2004) Histone H1-like protein participates in endothelial cell-specific activation of the von Willebrand factor promoter.Blood. 104(6): 1725-1732.
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