|Application ||IHC-P, IHC-F, E|
|Calculated MW||11672 Da|
|Purification||Antisera to pig insulin were raised by repeated immunisations of Guinea Pigs with highly purified antigen. Ig fraction prepared from whole serum by ammonium sulphate precipitation.|
|Shelf Life||18 months from date of despatch.|
|Other Names||Insulin, Insulin B chain, Insulin A chain, INS|
|Target/Specificity||Guinea pig anti-Pig insulin antibody recognizes porcine insulin, a hormone secreted by the beta cells of the islets of Langerhans in the pancreas. Insulin belongs to a family of hormones including the insulin-like growth hormones and the relaxins. The insulin precursor is a 108amino acid polypeptide consisting of a 24 aa signal peptide, an insulin A chain, a prohormone polypeptide (also known as the ‘C chain’) and an insulin B chain together having a predicted molecular mass of 11,672 Da. Active insulin migrates with an apparent molecular mass of 6kDa in western blotting of porcine, bovine and lapine samples (Schechteret al.1992).Insulin is a hormone that functions in carbohydrate homeostasis, and acts to decrease blood glucose concentration by increasing cell permeability to monosaccharides, amino acids and fatty acids, and accelerate glycolysis and glycogen synthesis in the liver, it induces uptake of glucose into cells through its interaction with the glucose transporter GLUT4 (Watson and Pressin 2001). Deficiency of insulin results in diabetes mellitus, one of the leading causes of morbidity and mortality in the general population. Insulin is also present in tumours of B-cell origin such as insulinoma (Schielet al.2006).Guinea pig anti-pig insulin antibody has been successfully used to demonstrate the presence og insulin in the islets of Langerhans of murine pancreas using immunohistochemistry on paraffin embedded material (Ortsäteret al.2012).|
|Preservative & Stabilisers||0.09% Sodium Azide|
|Storage||Store at +4℃ or at -20 ℃.|
|Precautions||Anti-Pig Insulin Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
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Provided below are standard protocols that you may find useful for product applications.
1. Ortsäter, H. et al. (2012) Diet supplementation with green tea extract epigallocatechin gallate prevents progression to glucose intolerance in db/db mice.Nutr Metab (Lond). 9: 11. 2. Izumi, K. et al. (2015) Reduced Tyk2 gene expression in ß-cells due to natural mutation determines susceptibility to virus-induced diabetes.Nat Commun. 6: 6748.
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