|Application ||WB, IHC-P, IF, E|
|Calculated MW||44106 Da|
|Purification||Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant|
|Immunogen||Human breast cancer cell line MCF-7.|
|Shelf Life||18 months from date of despatch.|
|Other Names||Keratin, type I cytoskeletal 19, Cytokeratin-19, CK-19, Keratin-19, K19, KRT19|
|Target/Specificity||Mouse anti-Human cytokeratin 19 antibody, clone A53-B/A2 recognizes the rod domain (aa 312-335) of human cytokeratin 19 (Böttger;et al.1995) also known as keratin 19 encoded by the KRT19 gene. Cytokeratin 19 is a 400 amino acid intermediate filament protein lacking a C-terminal tail domain, in contrast to all other intermediate filament proteins.Cytokeratin 19 expression is observed in striated muscle where it is involved in forming the association between the contractile apparatus and dystrophin (Stoneet al.2005). Expression is also seen in many ductal and glandular cells together with a restricted set of normal and neoplasic epithelial cells. Mouse anti-Human cytokeratin 19 antibody, clone A53-B/A2 detects a band of ~45 kDA in Western blotting using an A-549 human alveolar adenocarcinoma cell line lysate.|
|Preservative & Stabilisers||0.09% Sodium Azide (NaN3)|
|Storage||Store at +4℃ or at -20 ℃.|
|Precautions||Anti-Human Cytokeratin 19 Antibody, clone A53-B/A2 is for research use only and not for use in diagnostic or therapeutic procedures.|
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Provided below are standard protocols that you may find useful for product applications.
1. Karsten, U. et al. (1985) Monoclonal anti-cytokeratin antibody from a hybridoma clone generated by electrofusion.Eur. J. Cancer Clin. Oncol. 21 (6): 733-740. 2. Kasper, M. et al. (1987) Histological evaluation of three new monoclonal anti-cytokeratin antibodies. 1. Normal tissues.Eur. J. Cancer Clin. Oncol. 23(2): 137-147. 3. Goletz, S. et al (1997) Novel αGaINAc containing glycans on cytokeratins are recognized in vitro by galectins with type II carbohydrate recognition domains.J.Cell Science 110, 1585-1596. 4. de Neergaard, M. et al. (2010) Epithelial-stromal interaction 1 (EPSTI1) substitutes for peritumoral fibroblasts in the tumor microenvironment.Am J Pathol. 176: 1229-40.
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