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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   Anti-Human Cytokeratin 19 Antibody, clone A53-B/A2    

Anti-Human Cytokeratin 19 Antibody, clone A53-B/A2

Mouse Anti-Human Monoclonal Antibody

  • E - Anti-Human Cytokeratin 19 Antibody, clone A53-B/A2  ABD10601
    Sandwich ELISA analysis of human cytokeratin-19 expression using Mouse anti Human cytokeratin 19 antibody, clone BA19 as a capture reagent and biotinylated Mouse anti Human cytokeratin 19 antibody, clone A53-B/A2 (5552-9009
  • IHC-P - Anti-Human Cytokeratin 19 Antibody, clone A53-B/A2  ABD10601
    Staining of paraffin embedded human appendix with Mouse anti Human cytokeratin 19
  • WB - Anti-Human Cytokeratin 19 Antibody, clone A53-B/A2  ABD10601
    Western blot analysis of A549 human alveolar adenocarcinoma whole cell lysate probed with Mouse anti Human cytokeratin 19 antibody followed by HRP conjugated Goat anti Mouse IgG, visualized by chemiluminescence
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P08727
Reactivity Human
Host Mouse
Clonality Monoclonal
Isotype IgG2a
Clone Names A53-B/A2
Calculated MW 44106 Da
Additional Information
Purification Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Immunogen Human breast cancer cell line MCF-7.
Shelf Life 18 months from date of despatch.
Gene ID 3880
Other Names Keratin, type I cytoskeletal 19, Cytokeratin-19, CK-19, Keratin-19, K19, KRT19
Target/Specificity Mouse anti-Human cytokeratin 19 antibody, clone A53-B/A2 recognizes the rod domain (aa 312-335) of human cytokeratin 19 (Böttger;et al.1995) also known as keratin 19 encoded by the KRT19 gene. Cytokeratin 19 is a 400 amino acid intermediate filament protein lacking a C-terminal tail domain, in contrast to all other intermediate filament proteins.Cytokeratin 19 expression is observed in striated muscle where it is involved in forming the association between the contractile apparatus and dystrophin (Stoneet al.2005). Expression is also seen in many ductal and glandular cells together with a restricted set of normal and neoplasic epithelial cells. Mouse anti-Human cytokeratin 19 antibody, clone A53-B/A2 detects a band of ~45 kDA in Western blotting using an A-549 human alveolar adenocarcinoma cell line lysate.
Preservative & Stabilisers 0.09% Sodium Azide (NaN3)
Storage Store at +4℃ or at -20 ℃.
PrecautionsAnti-Human Cytokeratin 19 Antibody, clone A53-B/A2 is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
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1. Karsten, U. et al. (1985) Monoclonal anti-cytokeratin antibody from a hybridoma clone generated by electrofusion.
Eur. J. Cancer Clin. Oncol. 21 (6): 733-740. 2. Kasper, M. et al. (1987) Histological evaluation of three new monoclonal anti-cytokeratin antibodies. 1. Normal tissues.
Eur. J. Cancer Clin. Oncol. 23(2): 137-147. 3. Goletz, S. et al (1997) Novel αGaINAc containing glycans on cytokeratins are recognized in vitro by galectins with type II carbohydrate recognition domains.
J.Cell Science 110, 1585-1596. 4. de Neergaard, M. et al. (2010) Epithelial-stromal interaction 1 (EPSTI1) substitutes for peritumoral fibroblasts in the tumor microenvironment.
Am J Pathol. 176: 1229-40.

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Cat# ABD10601
(40 western blots)
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