|Application ||IHC-P, IHC-F, E|
|Calculated MW||122102 Da|
|Purification||Purified IgG prepared by affinity chromatography on Protein A|
|Immunogen||Human breast cancer cell line ZR-75-1.|
|Shelf Life||18 months from date of despatch.|
|Other Names||Mucin-1, MUC-1, Breast carcinoma-associated antigen DF3, Cancer antigen 15-3, CA 15-3, Carcinoma-associated mucin, Episialin, H23AG, Krebs von den Lungen-6, KL-6, PEMT, Peanut-reactive urinary mucin, PUM, Polymorphic epithelial mucin, PEM, Tumor-associated epithelial membrane antigen, EMA, Tumor-associated mucin, CD227, Mucin-1 subunit alpha, MUC1-NT, MUC1-alpha, Mucin-1 subunit beta, MUC1-beta, MUC1-CT, MUC1, PUM|
|Target/Specificity||Mouse anti-Human CD227 antibody, clone VU-11-E2recognises human Mucin-1 also known as CD227, Breast carcinoma-associated antigen DF3, Cancer antigen 15-3, Episialin, Krebs von den Lungen-6 or Tumor-associated epithelial membrane antigen. CD227 is a breast cancer associated mucin encoded by the MUC1 gene. Mucins are a family of high molecular weight, heavily glycosylated proteins (glycoconjugates) produced by many epithelial tissues in vertebrates. CD227 is expressed on most secretory epithelium, including mammary gland and some hematopoietic cells and is overexpressed abundantly in >90% of breast carcinomas and metastases.The epitope recognised by Mouse anti-Human CD227 antibody, clone VU-11-E2 is restricted to the 7-mer TSAPDTR of the CD227 tandem repeat.Mouse anti-Human CD227 antibody, clone VU-11-E2 has been tested on frozen human ovarian carcinoma tissue sections and paraffin embedded sections from normal breast tissue. A weak reaction is observed with sections of small intestine and colon.|
|Preservative & Stabilisers||0.09% Sodium Azide|
|Storage||Store at +4℃ or at -20 ℃.|
|Precautions||Anti-Human CD227 Antibody, clone VU-11E2 is for research use only and not for use in diagnostic or therapeutic procedures.|
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Provided below are standard protocols that you may find useful for product applications.
1. Cao, Y.et al (1998) Immunohistochemical characterization of a panel of 56 antibodies with normal human small intestine, colon, and breast tissues.Tumour Biol. 19: 88-99. 2. Price, M.et al (1998) Summary report on the ISOBM TD-4 Workshop: analysis of 56 monoclonal antibodies against the MUC1 mucin. San Diego, Calif., November 17-23, 1996.Tumour Biol, 19: 1-20. 3. Petrakou, E.et al (1998) Epitope mapping of anti-MUC1 mucin protein core monoclonal antibodies.Tumour Biol. 19: 21-9. 4. Schol, D.J,et al (1998) 'Epitope fingerprinting' using overlapping 20-mer peptides of the MUC1 tandem repeat sequence.Tumour Biol. 19: 35-45. 5. Karsten, U. et al. (2004) Binding patterns of DTR-specific antibodies reveal a glycosylation-conditioned tumor-specific epitope of the epithelial mucin (MUC1).Glycobiology. 14: 681-92.
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