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>   home   >   Products   >   Primary Antibodies   >   Stem Cells   >   Anti-Rat Nestin Antibody, clone Rat-401 (4D4)    

Anti-Rat Nestin Antibody, clone Rat-401 (4D4)

Mouse Anti-Rat Monoclonal Antibody

Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P21263
Reactivity Rat
Host Mouse
Clonality Monoclonal
Isotype IgG1
Clone Names Rat-401 (4D4)
Calculated MW 208797 Da
Additional Information
Other Species M
Immunogen Nestin purified from embryonic rat spinal cord.
Shelf Life 18 months from date of despatch.
Gene ID 25491
Other Names Nestin, Nes
Target/Specificity Mouse anti-Rat Nestin antibody, clone Rat-401 recognizes rat nestin, a large intermediate filament protein transiently expressed in embryonic glian cells (Hockfield and McKay 1985). It is predominately expressed in stem cells of the developing nervous system. Terminal differentiation is associated with a loss of nestin expression. Nestin expression has also been noted in other embryonic tissues, also in most Glioblastoma multiformes and many melanomas.
Preservative & Stabilisers 0.09% Sodium Azide (NaN3)
Storage Store at +4℃ or at -20 ℃.
PrecautionsAnti-Rat Nestin Antibody, clone Rat-401 (4D4) is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
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1. Hockfield, S. & McKay, R.D. (1985) Identification of major cell classes in the developing mammalian nervous system.
J Neurosci. 5 (12): 3310-28. 2. Arnold, T.D. et al. (2012) Defective Retinal Vascular Endothelial Cell Development As a Consequence of Impaired Integrin αVβ8-Mediated Activation of Transforming Growth Factor-β.
J Neurosci. 32: 1197-206. 3. Choi, J.S. et al. (2010) Expression of vascular endothelial growth factor receptor-3 mRNA in the rat developing forebrain and retina.
J Comp Neurol. 518: 1064-81. 4. Choi, J.S. et al. (2007) Upregulation of vascular endothelial growth factor receptors Flt-1 and Flk-1 following acute spinal cord contusion in rats.
J Histochem Cytochem. 55: 821-30. 5. Choi, J.S. et al. (2009) Transient expression of Bis protein in midline radial glia in developing rat brainstem and spinal cord.
Cell Tissue Res. 337: 27-36. 6. Shin, Y.J. et al. (2010) Enhanced expression of vascular endothelial growth factor receptor-3 in the subventricular zone of stroke-lesioned rats.
Neurosci Lett. 469: 194-8. 7. Zhang, H. et al. (2003) VEGF is a chemoattractant for FGF-2-stimulated neural progenitors.
J Cell Biol. 163: 1375-84. 8. Aleksandrova, M.A. et al. (2001) Transplantation of Cultured Human Neural Progenitor Cells into Rat Brain: Migration and Differentiation
Bull Exp Biol Med. 132: 1000-3. 9. Bertelli, E. et al. (2002) Nestin expression in rat adrenal gland.
Histochem Cell Biol. 117: 371-7. 10. Choi, J.S. et al. (2009) Enhanced expression of SOCS-2 in the rat hippocampus after transient forebrain ischemia.
J Neurotrauma. 26: 2097-106. 11. Poltavtseva, R.A. et al.(2001)In vitro development of neural progenitor cells from human embryos.
Bull Exp Biol Med. 132: 861-3. 12. Shin, Y.J. et al. (2013) Induction of vascular endothelial growth factor receptor-3 expression in perivascular cells of the ischemic core following focal cerebral ischemia in rats.
Acta Histochem. 115 (2): 170-7. 13. Barreira, A.L. et al. (2009) Bone marrow mononuclear cells attenuate interstitial fibrosis and stimulate the repair of tubular epithelial cells after unilateral ureteral obstruction.
Cell Physiol Biochem. 24: 585-94. 14. Mori, T. et al. (2005) Combination of hTERT and bmi-1, E6, or E7 induces prolongation of the life span of bone marrow stromal cells from an elderly donor without affecting their neurogenic potential.
Mol Cell Biol. 25: 5183-95.

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Cat# ABD10658
(40 western blots)
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