|Application ||WB, IHC-P, IHC-F, IF|
|Clone Names||Rat-401 (4D4)|
|Calculated MW||208797 Da|
|Immunogen||Nestin purified from embryonic rat spinal cord.|
|Shelf Life||18 months from date of despatch.|
|Other Names||Nestin, Nes|
|Target/Specificity||Mouse anti-Rat Nestin antibody, clone Rat-401 recognizes rat nestin, a large intermediate filament protein transiently expressed in embryonic glian cells (Hockfield and McKay 1985). It is predominately expressed in stem cells of the developing nervous system. Terminal differentiation is associated with a loss of nestin expression. Nestin expression has also been noted in other embryonic tissues, also in most Glioblastoma multiformes and many melanomas.|
|Preservative & Stabilisers||0.09% Sodium Azide (NaN3)|
|Storage||Store at +4℃ or at -20 ℃.|
|Precautions||Anti-Rat Nestin Antibody, clone Rat-401 (4D4) is for research use only and not for use in diagnostic or therapeutic procedures.|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
1. Hockfield, S. & McKay, R.D. (1985) Identification of major cell classes in the developing mammalian nervous system.J Neurosci. 5 (12): 3310-28. 2. Arnold, T.D. et al. (2012) Defective Retinal Vascular Endothelial Cell Development As a Consequence of Impaired Integrin αVβ8-Mediated Activation of Transforming Growth Factor-β.J Neurosci. 32: 1197-206. 3. Choi, J.S. et al. (2010) Expression of vascular endothelial growth factor receptor-3 mRNA in the rat developing forebrain and retina.J Comp Neurol. 518: 1064-81. 4. Choi, J.S. et al. (2007) Upregulation of vascular endothelial growth factor receptors Flt-1 and Flk-1 following acute spinal cord contusion in rats.J Histochem Cytochem. 55: 821-30. 5. Choi, J.S. et al. (2009) Transient expression of Bis protein in midline radial glia in developing rat brainstem and spinal cord.Cell Tissue Res. 337: 27-36. 6. Shin, Y.J. et al. (2010) Enhanced expression of vascular endothelial growth factor receptor-3 in the subventricular zone of stroke-lesioned rats.Neurosci Lett. 469: 194-8. 7. Zhang, H. et al. (2003) VEGF is a chemoattractant for FGF-2-stimulated neural progenitors.J Cell Biol. 163: 1375-84. 8. Aleksandrova, M.A. et al. (2001) Transplantation of Cultured Human Neural Progenitor Cells into Rat Brain: Migration and DifferentiationBull Exp Biol Med. 132: 1000-3. 9. Bertelli, E. et al. (2002) Nestin expression in rat adrenal gland.Histochem Cell Biol. 117: 371-7. 10. Choi, J.S. et al. (2009) Enhanced expression of SOCS-2 in the rat hippocampus after transient forebrain ischemia.J Neurotrauma. 26: 2097-106. 11. Poltavtseva, R.A. et al.(2001)In vitro development of neural progenitor cells from human embryos.Bull Exp Biol Med. 132: 861-3. 12. Shin, Y.J. et al. (2013) Induction of vascular endothelial growth factor receptor-3 expression in perivascular cells of the ischemic core following focal cerebral ischemia in rats.Acta Histochem. 115 (2): 170-7. 13. Barreira, A.L. et al. (2009) Bone marrow mononuclear cells attenuate interstitial fibrosis and stimulate the repair of tubular epithelial cells after unilateral ureteral obstruction.Cell Physiol Biochem. 24: 585-94. 14. Mori, T. et al. (2005) Combination of hTERT and bmi-1, E6, or E7 induces prolongation of the life span of bone marrow stromal cells from an elderly donor without affecting their neurogenic potential.Mol Cell Biol. 25: 5183-95.
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