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Anti-Human Neuropeptide Y Receptor 1 Antibody

Sheep Anti-Human Polyclonal Antibody

     
  • FC - Anti-Human Neuropeptide Y Receptor 1 Antibody  ABD10664
    Published customer image:Sheep anti Human neuropeptide Y receptor 1 antibody used for the detection of NY1R in rat retinal ganglion cells by immunogluorescence.Image caption:.NPY and NPY receptor immunoreactivity (ir) in a purified RGC culture obtained from Wistar pups (a–e, red). RGCs were stained with antiBrn3a (RGC marker, green) and nuclei with DAPI (blue). Scale bar: 20?μm.From: Martins J, Elvas F, Brudzewsky D, Martins T, Kolomiets B, Tralhão P, Gøtzsche CR, Cavadas C, Castelo-Branco M, Woldbye DP, Picaud S, Santiago AR, Ambrósio AF. Activation of Neuropeptide Y Receptors Modulates Retinal Ganglion Cell Physiologyand Exerts Neuroprotective Actions In Vitro.ASN Neuro. 2015 Aug 26;7. pii:1759091415598292.
  • WB - Anti-Human Neuropeptide Y Receptor 1 Antibody  ABD10664
    Human brain lysate probed with Sheep anti Human Neuropeptide Y Receptor 1
  • IF - Anti-Human Neuropeptide Y Receptor 1 Antibody  ABD10664
    Published customer image:Sheep anti Human neuropeptide Y receptor 1 antibody used for the detection of NY1R in rat retinal ganglion cells by immunogluorescence.Image caption:.NPY-ir and NPY receptor-ir in retinal slices obtained from young adult Wistar rats (a–e, red). RGCs were stained with the RGC marker Brn3a and nuclei with DAPI . NPY-ir was mainly detected in strata 1, 3, and 5 of IPL (arrows; a). Y1-ir was detected in distal and proximal INL, strata 2 and 4 of IPL, and RGCs (arrows; b). Y2-ir was detected in INL (c). Y4-ir was detected in INL and GCL (d). and Y5-ir was detected in Müller cells (e). (d) Y5-ir is colocalized with Müller cells in retinal slices of young adult Wistar rats. Müller cells were identified by vimentin-ir . Nuclei were stained with DAPI . GCL?=?ganglion cell layer; IPL?=?inner plexiform layer; INL?=?inner nuclear layer; OPL?=?outer plexiform layer; ONL?=?outer nuclear layer. Scale bar: 50?μm.From: Martins J, Elvas F, Brudzewsky D, Martins T, Kolomiets B, Tralhão P, Gøtzsche CR, Cavadas C, Castelo-Branco M, Woldbye DP, Picaud S, Santiago AR, Ambrósio AF. Activation of Neuropeptide Y Receptors Modulates Retinal Ganglion Cell Physiologyand Exerts Neuroprotective Actions In Vitro.ASN Neuro. 2015 Aug 26;7(4). pii:1759091415598292.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, E
Primary Accession P25929
Reactivity Human
Host Sheep
Clonality Polyclonal
Isotype Polyclonal IgG
Calculated MW 44392 Da
Additional Information
Other Species Rat
Purification Purified IgG prepared by affinity chromatography on Protein G
Immunogen A synthetic peptide corresponding to amino acids 365 - 378 of human NPY1R, conjugated to KLH.Peptide sequence: KQASPVAFKKINNN
Shelf Life 18 months from date of despatch.
Gene ID 4886
Other Names Neuropeptide Y receptor type 1, NPY1-R, NPY1R, NPYR, NPYY1
Target/Specificity Sheep anti-Human Neuropeptide Y Receptor 1 antibody recognizes human neuropeptide Y receptor 1. Neuropeptide Y receptors are a class of G-protein coupled receptors which are activated by the closely related peptide hormones neuropeptide Y, peptide YY and pancreatic polypeptide. These receptors are involved in control of a diverse set of behavioral processes including appetite, circadian rhythm, and anxiety.
Preservative & Stabilisers 0.09% Sodium Azide (NaN3)
Storage Store at +4℃ or at -20 ℃.
PrecautionsAnti-Human Neuropeptide Y Receptor 1 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
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References

1. Ferreira, R. et al. (2010) Neuropeptide Y Modulation of Interleukin-1{beta} (IL-1{beta})-induced Nitric Oxide Production in Microglia.
J Biol Chem. 285: 41921-34. 2. Decressac, M. et al. (2009) Neuropeptide Y stimulates proliferation, migration and differentiation of neural precursors from the subventricular zone in adult mice.
Neurobiol Dis. 34: 441-9. 3. Martins, J. et al. (2015) Activation of Neuropeptide Y Receptors Modulates Retinal Ganglion Cell Physiology and Exerts Neuroprotective Actions In Vitro.
ASN Neuro. 7 (4) pii: 1759091415598292.

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Cat# ABD10664
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