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Anti-Peptidoglycan Antibody, clone 3F6B3 (10H6) (Ascites)

Mouse Anti-Bacterial Monoclonal Antibody

  • IF - Anti-Peptidoglycan Antibody, clone 3F6B3 (10H6) (Ascites) ABD10692
    Published customer image:Mouse anti Peptidoglycan antibody used to detect spirochetes in the brain of a patient with Lyme neuroborreliosis by immunofluorescence.Extra- and intracellular atypical and cystic forms of spirochetes in the cerebral cortex of a patient with pathologically and serologically confirmed chronic Lyme neuroborreliosis where Borrelia burgdorferi sensu stricto was cultivated from the brain. A: Colony-like agglomeration of spirochetes as revealed by monoclonal anti-OspA antibody in the cerebral cortex. B: A close up of the central part of the mass seen in A. In addition to a few helically shaped spirochetes (arrow) numerous ring-shaped forms and spherules (asterisk) are visible, which are identical to those observed in vitro following 1 week Borrelia exposure of primary neurons (compare with Fig. 4G). C: Spirochetes showing loop or ring-shaped formations (arrows) in the cerebral cortex immunostained with a polyclonal anti-Borrelia burgdorferi antibody (Biodesign, B65302R). They are similar to those of Treponema pallidum (arrows in D) observed in the cerebral cortex of a patient with general paresis. Immunostaining was performed using a polyclonal anti-Treponema pallidum antibody (Biodesign, B65210R). E: Helically shaped OspA immunoreactive spirochetes in the cytoplasm of a cortical pyramidal neuron. In addition to one more typical form (arrow), fine OspA positive minute granules along filamentous forms are seen. F: Intracellular ring-shaped forms (arrow) showing positive immunoreaction with a polyclonal anti-Borrelia burgdorferi antibody . They are identical to those observed in chicken primary neurons infected with Borrelia (compare F with Figure 4D). Near the asterisk a large strongly immunoreactive cyst form is visible. Spirochete forming loop in the cerebral cortex (G) and in the cytoplasm of an epithelial cell of the choroid plexus (H) are seen as visualized by anti-OspA and anti-bacterial peptidoglycan antibodies, respectively. I: A similar atypical spirochete forming loops in the cerebral cortex as visualized with Thioflavin S. J: In an area with colony-like spirochete aggregation in addition to some typical, regularly coiled Borrelia spirochetes (arrow) OspA positive cystic forms (asterisk) are seen. K: In the cerebral cortex near the colony-like spirochetal agglomerate a spirochete cyst (asterisk) similar to that observed in vitro is visible (compare it with Figure 5 G-J). Immunostaining was performed using a monoclonal anti-OspA antibody. Bars: A = 20 μm; B-J = 10 μm, K = 5 μm.From: Miklossy J, Kasas S, Zurn AD, McCall S, Yu S, McGeer PL.Persisting atypical and cystic forms of Borrelia burgdorferi and local inflammation in Lyme neuroborreliosis.J Neuroinflammation. 2008 Sep 25;5:40.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Reactivity Bacteria
Host Mouse
Clonality Monoclonal
Isotype IgG1
Clone Names 3F6B3 (10H6)
Additional Information
Immunogen This antibody was raised against insoluble peptidoglycan obtained by TCA-heat and ethanol extraction of Streptococcus mutans BHT cells.
Shelf Life 18 months from date of despatch.
Target/Specificity Mouse anti-Peptidoglycan antibody, clone 3F6B3 recognizes the 3D polymer complex structure of peptidoglycan (PG). In a competitive immunoassay format, several compounds were found to be ineffective as inhibitors; muramyldipeptide, N-acetylglucosamine, chitin and acid hydrolyzed chitin. The epitope appears to consist of discontinuous glycan and/or amino acid residues.
Preservative & Stabilisers Preservative Free
Storage Store at -20℃ only.
PrecautionsAnti-Peptidoglycan Antibody, clone 3F6B3 (10H6) (Ascites) is for research use only and not for use in diagnostic or therapeutic procedures.
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1. Miklossy, J. et al(2004)Borrelia burgdorferi persists in the brain in chronic lyme neuroborreliosis and may be associated with Alzheimer disease.
J. Alzheimer's Dis. 6: 639-649. 2. Wu, L. et al. (2007) Bacterial peptidoglycan breaks down intestinal tolerance via mast cell activation: the role of TLR2 and NOD2.
Immunol Cell Biol. 85: 538-45. 3. Rennemeier, C. et al. (2007) Thrombospondin-1 promotes cellular adherence of gram-positive pathogens via recognition of peptidoglycan.
FASEB J. 21 (12): 3118-32. 4. Schweitzer, M.H. et al. (2016) Testing the Hypothesis of Biofilm as a Source for Soft Tissue and Cell-Like Structures Preserved in Dinosaur Bone.
PLoS One. 11 (2): e0150238. 5. Miklossy J et al. (2008) Persisting atypical and cystic forms of Borrelia burgdorferi and local inflammation in Lyme neuroborreliosis.
J Neuroinflammation. 5: 40.

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Cat# ABD10692
(40 western blots)
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