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>   home   >   Products   >   Primary Antibodies   >   Stem Cells   >   Anti-Human CD140b Antibody, clone PR7212    

Anti-Human CD140b Antibody, clone PR7212

Mouse Anti-Human Monoclonal Antibody

Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P09619
Reactivity Human
Host Mouse
Clonality Monoclonal
Isotype IgG1
Clone Names PR7212
Calculated MW 123968 Da
Additional Information
Other Species Baboon,Pr
Purification Purified IgG prepared by affinity chromatography on Protein G
Immunogen Human skin fibroblast cell membrane extracts.
Shelf Life 18 months from date of despatch.
Gene ID 5159
Other Names Platelet-derived growth factor receptor beta, PDGF-R-beta, PDGFR-beta,, Beta platelet-derived growth factor receptor, Beta-type platelet-derived growth factor receptor, CD140 antigen-like family member B, Platelet-derived growth factor receptor 1, PDGFR-1, CD140b, PDGFRB, PDGFR, PDGFR1
Target/Specificity Mouse anti-Human CD140b antibody, clone PR7212 recognizes the extracellular domain of the human PDGF receptor beta subunit. This was confirmed in binding studies using several different cell lines and by its ability to immunoprecipitate PDGF receptor beta complexed with 125I-PDGF-BB. Mouse anti-Human CD140b antibody, clone PR7212 does not recognize PDGF receptor alpha. PDGF receptor beta is a member of the class III receptor tyrosine kinase family that also includes M-CSF receptor, SCF receptor and Flt-3. Binding of PDGF-BB induces receptor homodimerization or heterodimerization with PDGF receptor alpha.
Preservative & Stabilisers 0.09% Sodium Azide (NaN3)
Storage Store at +4℃ or at -20 ℃.
PrecautionsAnti-Human CD140b Antibody, clone PR7212 is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
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1. Hart, C.E. et al. (1987) Synthesis, phosphorylation, and degradation of multiple forms of the platelet-derived growth factor receptor studied using a monoclonal antibody.
J Biol Chem. 262: 10780-5. 2. Hart, C.E. et al. (1988) Two classes of PDGF receptor recognize different isoforms of PDGF.
Science. 240: 1529-31. 3. Franklin, W.A. et al. (1990) In situ distribution of the beta-subunit of platelet-derived growth factor receptor in nonneoplastic tissue and in soft tissue tumors.
Cancer Res. 50: 6344-8. 4. Seifert, R.A. et al. (1989) Two different subunits associate to create isoform-specific platelet-derived growth factor receptors.
J Biol Chem. 264: 8771-8. 5. Krane, J.F. et al. (1991) Increased dermal expression of platelet-derived growth factor receptors in growth-activated skin wounds and psoriasis.
J Invest Dermatol. 96: 983-6. 6. Palman, C. et al. (1992) Platelet-derived growth factor receptor (beta-subunit) immunoreactivity in soft tissue tumors.
Lab Invest. 66: 108-15. 7. Ascoli, V. et al. (1995) Platelet-derived growth factor receptor immunoreactivity in mesothelioma and nonneoplastic mesothelial cells in serous effusions.
Acta Cytol. 39: 613-22. 8. Gilbertson, D.G. et al. (2001) Platelet-derived growth factor C (PDGF-C), a novel growth factor that binds to PDGF alpha and beta receptor.
J Biol Chem. 276: 27406-14. 9. Wang, J. et al. (1994) Cell proliferation in human soft tissue tumors correlates with platelet-derived growth factor B chain expression: an immunohistochemical and in situ hybridization study.
Cancer Res. 54: 560-4. 10. Meng, J. et al. (2011) Contribution of human muscle-derived cells to skeletal muscle regeneration in dystrophic host mice.
PLoS One. 6: e17454.

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Cat# ABD10699
(40 western blots)
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