|Application ||WB, IHC-P, E|
|Calculated MW||24824 Da|
|Purification||Purified IgG prepared by affinity chromatography on Protein G|
|Immunogen||Native, from brain|
|Shelf Life||18 months from date of despatch.|
|Other Names||Ubiquitin carboxyl-terminal hydrolase isozyme L1, UCH-L1, 22.214.171.124, 6.-.-.-, Neuron cytoplasmic protein 9.5, PGP 9.5, PGP9.5, Ubiquitin thioesterase L1, UCHL1|
|Target/Specificity||Mouse anti-Human Protein Gene Product 9.5 antibody, clone 31A3 recognizes protein gene product 9.5 (PGP9.5), a ubiquitin hydrolase which is widely expressed in neuronal tissues and represents 1-2% of total soluble brain proteins. PGP9.5, also known as ubiquitin C-terminal hydrolase 1 (UCHL-1), is involved in the regulation of the ubuiquitin pathway.Mouse anti-Human Protein Gene Product 9.5 antibody, clone 31A3 stains neuronal cell bodies and axons in the CNS and periphery, small nerve fibres in peripheral tissues, neuroendocrine cells in the pituitary, thyroid, pancreas and tumours of the DNES.Clones 31A3 and 13C4 each recognise a different epitope towards the N-terminus of the protein. Mouse anti-Human Protein Gene Product 9.5 antibody, clone 31A3 also recognizes PGP9.5 in other species, including rat and rabbit but evidence suggests it does not bind to PGP9.5 in guinea pigs (Wilsonet al.1988). Mouse anti-Human Protein Gene Product 9.5 antibody, clone 31A3 has been used successfully as a capture reagent with clone 13C4 as a detection reagent in a sandwich ELISA to evaluate contamination of processed meat samples with neuronal tissue (Gaunitzet al.2009).|
|Preservative & Stabilisers||0.09% Sodium Azide (NaN3)|
|Storage||Store at +4℃ or at -20 ℃.|
|Precautions||Anti-Human Protein Gene Product 9.5 Antibody, clone 31A3 is for research use only and not for use in diagnostic or therapeutic procedures.|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
1. Day, I.N.M and Thompson, R.J. (1986) Monoclonal antibodies to the neuroendocrine protein PGP 9.5Biochemical Society Transactions 14: 350–1. 3. García-Añoveros, J. et al. (2001) Transport and localization of the DEG/ENaC ion channel BNaC1alpha to peripheral mechanosensory terminals of dorsal root ganglia neurons.J Neurosci. 21 (8): 2678-86. 4. Verhein, K.C. et al. (2011) Three days after a single exposure to ozone, the mechanism of airway hyperreactivity is dependent on substance P and nerve growth factor.Am J Physiol Lung Cell Mol Physiol. 300: L176-84. 5. Caballero, O.L. et al. (2002) Interaction and colocalization of PGP9.5 withJAB1 and p27(Kip1).Oncogene. 21:3003-10. 6. Kondo, T. et al. (2013) Prostaglandin E2 mediates acid-induced heartburn in healthy volunteers.Am J Physiol Gastrointest Liver Physiol. 304(6):G568-73 7. Mihara, H. et al. (2010) Involvement of TRPV2 activation in intestinal movement through nitric oxideproduction in mice.J Neurosci. 30: 16536-44. 8. Forsgren, K. et al. (1999) Regeneration of nerve fibres in the maxillary sinus mucosa after experimental surgery. An immunocytochemical double-labelling study in the rabbit.Acta Otolaryngol. 119: 486-91. 9. Yee, C.L. et al. (2001)"Type III"cells of rat taste buds: immunohistochemical and ultrastructural studies of neuron-specific enolase, protein gene product 9.5, and serotonin.J Comp Neurol. 440: 97-108. 10. Pauza, D.H. et al. (2014) A combined acetylcholinesterase and immunohistochemical method for precise anatomical analysis of intrinsic cardiac neural structures.Ann Anat. pii: S0940-9602(14)00159-9. 11. Sugimoto, K. et al. (2011) Olmesartan ameliorates peripheral nerve dysfunction in Zucker diabetic fatty rats.J Hypertens. 29: 1337-46. 12. Bishop, P. et al. (2014) The ubiquitin C-terminal hydrolase L1 (UCH-L1) C terminus plays a key role in protein stability, but its farnesylation is not required for membrane association in primary neurons.J Biol Chem. 289 (52): 36140-9. 13. Moldovan, M. et al. (2013) Peptide mimetic of the S100A4 protein modulates peripheral nerve regeneration and attenuates the progression of neuropathy in myelin protein P0 null mice.Mol Med. 19: 43-53. 14. Nonclercq, D. et al. (2002) Phenotypic variations and dynamic topography of transformed cells in an experimental model of diethylstilbestrol-induced renal tumour in male Syrian hamster.Histochem J. 34 (10): 487-97. 15. Silva, I. et al. (2015) Activation of P2Y6 Receptors Facilitates Nonneuronal Adenosine Triphosphate and Acetylcholine Release from Urothelium with the Lamina Propria of Men with Bladder Outlet Obstruction.J Urol. 194 (4): 1146-54. 16. Gaunitz C et al. (2009) Suitability of antigens PGP 9.5 and neurofilament light as marker proteins for detection of neuronal tissue in processed meat products.J Food Prot. 72 (5): 1070-7. 17. Forsgren, K. et al. (1999) Regeneration of nerve fibres in the maxillary sinus mucosa after experimental surgery. An immunocytochemical double-labelling study in the rabbit.Acta Otolaryngol. 119 (4): 486-91. 18. Pauziene N et al. (2015) Innervation of the rabbit cardiac ventricles.J Anat. Oct 29. [Epub ahead of print] 19. Rashwan, A. et al. (2015) Ontogeny and innervation of taste buds in mouse palatal gustatory epithelium.J Chem Neuroanat. Dec 12 [Epub ahead of print]
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