|Application ||WB, E|
|Calculated MW||58048 Da|
|Purification||Ig fraction prepared by a multi-step process including delipidation, salt fractionation and ion exchange chromatography followed by dialysis.|
|Immunogen||Pyruvate kinase from rabbit muscle.|
|Shelf Life||18 months from date of despatch.|
|Other Names||Pyruvate kinase PKM, 188.8.131.52, Pyruvate kinase muscle isozyme, PKM, PKM2|
|Target/Specificity||Pyruvate kinase is an enzyme invloved in glycolysis that catalyzes the transfer of phosphate from phosphoenol pyruvate (PEP) to ADP yielding ATP and pyruvate. Pyruvate can then be converted to Acetyl CoA and enter the TCA cycle. At least three molecular forms with pyruvate kinase activity are known. PK1, the red cell form, is deficient in a type of hemolytic anemia. PK2 is found in kidney. PK3 is found in leukocytes, muscle, platelets, and brain but not in red cells or kidney.Assay by IEP gives a single precipitin arc against anti-goat serum as well as partially purified rabbit muscle pyruvate kinase. This antibody has been reported to react with all forms of pyruvate kinase.|
|Preservative & Stabilisers||0.09% Sodium Azide (NaN3)|
|Storage||Store at +4℃ or at -20 ℃.|
|Precautions||Anti-Pyruvate Kinase Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
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