|Application ||IHC-F, IF|
|Other Species||H,Rat,Goldfish,Cat,C,Marine file snake,Helix aspersa,Hirudo medicinalis|
|Immunogen||Serotonin conjugated to bovine serum albumin.|
|Shelf Life||18 months from date of despatch.|
|Target/Specificity||Rat anti-serotonin antibody, clone YC5/45 recognizes serotonin and does not cross react with 5-hydroxyindolacetic acid, GABA, noradrenaline, 5-hydroxytryptophan, carnosine or melatonin.Serotonin, also known as 5-hydroxytryptamine or 5-HT is a biochemical messenger and neurotransmitter synthesized from L-tryptophan. Serotonin has a range of functions, including regulation of gastric motility, and may be related to some psychiatric disorders including schizophrenia and depression. Serotonin is widely distributed in the central and peripheral nervous system, and also in some non-neural tissues such as platelets.Serotonin appears widely conserved and Rat anti-serotonin antibody, clone YC5/45 has been used successfully to demonstrate serotonin immunoreactiviy using immunohistochemistry in many diverse groups including primates (Vanoliet al.2013), rodents (Hosodaet al.1984), prototherian mammals, avians and reptiles (Adamson and Campbell 1988), teleosts (Takedaet al.2014) through to invertebrates including hirudinids (Masuda-Nakagawaet al.1990) and gastropods (Osborne and Dockray 1982).|
|Preservative & Stabilisers||0.05% Thiomersal;|
|Storage||Store at +4℃ or at -20 ℃.|
|Precautions||Anti-Serotonin Antibody, clone YC5/45 is for research use only and not for use in diagnostic or therapeutic procedures.|
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Provided below are standard protocols that you may find useful for product applications.
1. Consolazione, A. et al. (1981) Immunocytochemical detection of serotonin with monoclonal antibodies.J Histochem Cytochem. 29: 1425-30. 2. Milstein, C. et al. (1983) The discrepancy between the cross-reactivity of a monoclonal antibody to serotonin and its immunohistochemical specificity.Mol. Immunol. 20(1): 113-123. 3. Suárez-Quian, C.A. (1987) The distribution of four lysosomal integral membrane proteins (LIMPs) in rat basophilic leukemia cells.Tissue Cell. 19: 495-504. 4. La Rosa, S. et al. (2011) Histologic characterization and improved prognostic evaluation of 209 gastric neuroendocrine neoplasms.Hum Pathol. 42: 1373-84. 5. Takeda, A. et al. (2014) Axonal regeneration through the fibrous scar in lesioned goldfish spinal cord.Neuroscience. pii: S0306-4522(14)00826-4. 6. Cuello, A.C. et al. (1982) Immunocytochemistry with internally labeled monoclonal antibodies.Proc Natl Acad Sci U S A. 79: 665-9. 7. Gil-Loyzaga, P. et al. (1997) Serotonergic innervation of the organ of Corti of the cat cochlea.Neuroreport. 8: 3519-22. 8. Osborne, N.N. (1982) Uptake, localization and release of serotonin in the chick retina.J Physiol. 331: 469-79. 9. Vanoli, A. et al. (2013) Histologic changes in type A chronic atrophic gastritis indicating increased risk of neuroendocrine tumor development: the predictive role of dysplastic and severely hyperplastic enterochromaffin-like cell lesions.Hum Pathol. 44: 1827-37. 10. Dainese, E. et al. (2013) Linear and micronodular neuroendocrine cell hyperplasia in an ovarian mucinous cystadenoma.Pathol Res Pract. 209: 670-3.
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