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Anti-Toxoplasma gondii RH Strain Antibody (Biotin)

  • IF - Anti-Toxoplasma gondii RH Strain Antibody (Biotin) ABD10804
    Published customer image:Rabbit anti Toxoplasma gondii RH Strain antibody used for the identification of Toxoplasma cysts by immunofluorescence.Image caption:Compound 2 enhances differentiation. A. Structure of Compound 2. B. Immunofluorescence of a cyst-like structure using an anti-Toxoplasma antibody (green) to detect all parasites and Dolichos biflorus lectin (DL; red) to detect the cyst wall after 72 hr under CO2 starvation conditions. The corresponding differential interference contrast (DIC) and merged images are also shown. Scale bar = 10&mu;m. C. Percentage of DL-positive vacuoles (DL+) in samples treated with either Compound 2 (3&mu;M) or an equivalent volume of DMSO for 72 hr under CO2 starvation conditions (mean ± SD; n = 4 for the wild-type strain and n = 6 for &DELTA;uprt strains). The data were compared using paired Student&#39;s t-test (**p < 0.01).From: Odell AV, Tran F, Foderaro JE, Poupart S, Pathak R, Westwood NJ, et al. Yeast Three-Hybrid Screen Identifies TgBRADIN/GRA24 as a Negative Regulator of Toxoplasma gondii Bradyzoite Differentiation.PLoS ONE 10: e0120331.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Host Rabbit
Clonality Polyclonal
Isotype Polyclonal IgG
Additional Information
Purification Purified Ig prepared by affinity chromatography on Protein A
Immunogen Toxoplasma gondii RH strain.
Shelf Life 18 months from the date of despatch.
Target/Specificity Rabbit anti-Toxoplasma gondii RH Strain antibody recognizes purified Toxoplasma gondiitrophozoites.T. gondii is an intracellular protozoan parasite that enters its host via the gastrointestinal tract, and is the only member of the Toxoplasma genus. The definitive host of T. gondii is cats, but it can also be carried by a vast number of warm-blooded animals, including humans. Toxoplasmosis is usually minor and self-limiting but can have serious or even fatal effects on a fetus, or if the host is immunocompromised. T. gondii encephalitis (TE) is the most common cerebral opportunistic infection in patients with AIDS.
Preservative & Stabilisers 0.09% Sodium Azide
Storage Store at +4℃ or at -20 ℃.
PrecautionsAnti-Toxoplasma gondii RH Strain Antibody (Biotin) is for research use only and not for use in diagnostic or therapeutic procedures.
Citations (0)

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1. Parussini, F. et al. (2012) Intramembrane proteolysis of Toxoplasma apical membrane antigen 1 facilitates host-cell invasion but is dispensable for replication.
Proc Natl Acad Sci U S A. 109 (19): 7463-8. 2. Odell, A.V. et al. (2015) Yeast Three-Hybrid Screen Identifies TgBRADIN/GRA24 as a Negative Regulator of Toxoplasma gondii Bradyzoite Differentiation.
PLoS One. 10 (3): e0120331. 3. Passeri, E. et al. (2015) Infection and characterization of Toxoplasma gondii in human induced neurons from patients with brain disorders and healthy controls.
Microbes Infect. pii: S1286-4579(15)00207-5.

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Cat# ABD10804
(40 western blots)
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