|Application ||WB, IF, E, Func|
|Calculated MW||77064 Da|
|Purification||Purified IgG prepared by affinity chromatography on Protein A|
|Immunogen||Purified porcine transferrin|
|Shelf Life||18 months from date of despatch.|
|Other Names||Serotransferrin, Transferrin, Beta-1 metal-binding globulin, Siderophilin, TF|
|Target/Specificity||anti-transferrin clone HTF-14 is specific for the N-terminal domain of human transferrin and has been shown to block binding of transferrin to the transferrin receptor (CD71). Clone HTF-14 is not able to recognise receptor bound transferrin. Clone HTF-14 recognises a conformational epitope in a loop of the N lobe of transferrin involving the critical Lys-144 residue, also essential for binding of the N lobe to the transferrin receptor (Masonet al.2009)Transferrin is a 77 kDa plasma protein synthesized by the liver involved with the transport of iron. Each transferrin molecule has two domains, both with facility to carry 2 ferric ions. The iron/transferrin complex is essential for haemoglobin synthesis and certain types of cell division.|
|Preservative & Stabilisers||0.09% Sodium Azide|
|Storage||Store at +4℃ or at -20 ℃.|
|Precautions||Anti-Human Transferrin (N-Terminal) Antibody, clone HTF-14 is for research use only and not for use in diagnostic or therapeutic procedures.|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
1. Rubikaite, B.I. et al. (1989) [Identification of the segment for binding of transferrin using a cellular receptor].Mol Biol (Mosk). 23: 765-71. 2. Hradilek, A. and Neuwirt, J. (1986) Iron uptake by MOLT 3 cells from transferrin/monoclonal antitransferrin antibody complexes.Br J Haematol. 62: 21-30. 3. Trebichavsky, I. et al. (1987) Monoclonal antibodies against pig transferrin. Blocking and binding activity.Folia Microbiol (Praha). 32: 448-52. 4. Fuchs, O. et al. (1988) Non-transferrin donors of iron for heme synthesis in immature erythroid cells.Biochim Biophys Acta. 969: 158-65. 5. Bártek, J. et al. (1985) Phylogenetically more conservative epitopes among monoclonal antibody-defined antigenic sites of human transferrin are involved in receptor binding.Br J Haematol. 59: 435-41. 6. Penhallow, R.C. et al. (1986) Comparative studies of the binding and growth-supportive ability of mammalian transferrins in human cells.J Cell Physiol. 128: 251-60. 7. Teh, E.M. et al. (2007) Identification of the epitope of a monoclonal antibody that disrupts binding of human transferrin to the human transferrin receptor.FEBS J. 272: 6344-53. 8. Funk, W.D. et al. (1990) Expression of the Amino-Terminal Half-Molecule of Human Serum Transferrin in Cultured Cells and Characterization of the Recombinant ProteiBiochemistry. 29: 1654-60.
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