|Application ||WB, IHC-F|
|Calculated MW||51118 Da|
|Purification||Purified Ig prepared by affinity chromatography|
|Immunogen||Recombinant rabbit tryptophan hydroxylase, isolated as inclusion bodies from E. coli and purified by preparative SDS-PAGE.|
|Shelf Life||18 months from date of despatch.|
|Other Names||Tryptophan 5-hydroxylase 1, 18.104.22.168, Tryptophan 5-monooxygenase 1, TPH1, TPH|
|Target/Specificity||Sheep anti-Rabbit tryptophan hydroxylase antibody recognises tryptophan hydroxylase, also known as Tryptophan 5-hydroxylase 1 or Tryptophan 5-monooxygenase 1. Tryptophan hydroxylase is a 444 amino acid ~55 kDa enzyme involved in the synthesis of the neurotransmitter serotonin.|
|Preservative & Stabilisers||0.09% Sodium Azide (NaN3)|
|Storage||Store at +4℃ or at -20 ℃.|
|Precautions||Anti-Rabbit Tryptophan Hydroxylase Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
1. Lowry, A. et al (2000) Corticotropin-Releasing Factor Increases In Vitro Firing Rates of Serotonergic Neurons in the Rat Dorsal Raphe Nucleus: Evidence for Activation of a Topographically Organized Mesolimbocortical Serotonergic System.J. Neurosci 20: 7728 - 7736 2. Federici, L.M. et al. (2015) Hypothalamic orexin's role in exacerbated cutaneous vasodilation responses to an anxiogenic stimulus in a surgical menopause modelPsychoneuroendocrinology. 18 Dec. [Epub ahead of print] 3. Johnson, P.L. et al. (2005) Acute hypercarbic gas exposure reveals functionally distinct subpopulations of serotonergic neurons in rats.J Psychopharmacol. 19 (4): 327-41. 4. Sajdyk, T.J. et al. (2008) Neuropeptide Y in the amygdala induces long-term resilience to stress-induced reductions in social responses but not hypothalamic-adrenal-pituitary axis activity or hyperthermia.J Neurosci. 28 (4): 893-903. 5. Johnson, P. et al. (2008) Disruption of GABAergic tone in the dorsomedial hypothalamus attenuates responses in a subset of serotonergic neurons in the dorsal raphe nucleus following lactate-induced panic.J Psychopharmacol. 22 (6): 642-52. 6. Yu, B. et al. (2015) Mechanisms Underlying Footshock and Psychological Stress-Induced Abrupt Awakening From Posttraumatic"Nightmares".Int J Neuropsychopharmacol. pii: pyv113. 7. Abrams, J.K. et al. (2005) Serotonergic systems associated with arousal and vigilance behaviors following administration of anxiogenic drugs.Neuroscience. 133 (4): 983-97. 8. Staub, D.R. et al. (2005) Urocortin 2 increases c-Fos expression in topographically organized subpopulations of serotonergic neurons in the rat dorsal raphe nucleus.Brain Res. 1044 (2): 176-89. 9. Cui, S.Y. et al. (2011) Diltiazem potentiates pentobarbital-induced hypnosis via 5-HT1A and 5-HT2A/2C receptors: role for dorsal raphe nucleus.Pharmacol Biochem Behav. 99 (4): 566-72. 10. Bouwknecht, J.A. et al. (2007) Differential effects of exposure to low-light or high-light open-field on anxiety-related behaviors: relationship to c-Fos expression in serotonergic and non-serotonergic neurons in the dorsal raphe nucleus.Brain Res Bull. 72 (1): 32-43. 11. Gardner, K.L. et al. (2005) Early life experience alters behavior during social defeat: focus on serotonergic systems.Neuroscience. 136 (1): 181-91. 12. Hale, M.W. et al. (2008) Exposure to an open-field arena increases c-Fos expression in a subpopulation of neurons in the dorsal raphe nucleus, including neurons projecting to the basolateral amygdaloid complex.Neuroscience. 157 (4): 733-48. 13. Spiga, F. et al. (2006) Injections of urocortin 1 into the basolateral amygdala induce anxiety-like behavior and c-Fos expression in brainstem serotonergic neurons.Neuroscience. 138 (4): 1265-76. 14. Rook, G. et al. (2003) Treatment of conditions of the central nervous system using mycobacteria U.S. Patent Publication US20030170275 A1 15. Staub, D.R. et al. (2006) Evidence supporting a role for corticotropin-releasing factor type 2 (CRF2) receptors in the regulation of subpopulations of serotonergic neurons.Brain Res. 1070 (1): 77-89. 16. Lowry, C.A. et al. (2007) Identification of an immune-responsive mesolimbocortical serotonergic system: potential role in regulation of emotional behavior.Neuroscience. 146 (2): 756-72.
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