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Anti-Rabbit Tryptophan Hydroxylase Antibody

Sheep Anti-Rabbit Polyclonal Antibody

     
  • IF - Anti-Rabbit Tryptophan Hydroxylase Antibody  ABD10815
    Published customer image:Sheep anti Rabbit tryptophan hydroxylase antibody used for the demonstration of immunoreactive neurons in rat brain by immunofluorescence on cryostat sections.Image caption:Double immunofluorescence photomicrograph illustrating a retrogradely labeled serotonergic neuron in the DRD region of the mid-rostrocaudal dorsal raphe nucleus (-8.18 mm Bregma) following unilateral intra-BL injection of CTb. A) Photomicrograph showing the mid-rostrocaudal dorsal raphe nucleus. White box in A indicates region shown at higher magnification in B. White box in B indicates region shown at higher magnification in C, D and E. TrpOH-ir (serotonergic) neurons appear red (C) while CTb-ir (retrogradely labeled) neuron appears green (D). Double TrpOH/CTb immunofluorescent neuron appears yellow (E). Scale bar (A) 200μm, (B) 80μm, (C), (D) & (E) 20μm.From: Hale MW, Hay-Schmidt A, Mikkelsen JD, Poulsen B, Bouwknecht JA, Evans AK, Stamper CE, Shekhar A, Lowry CA. Exposure to an open-field arena increases c-Fos expression in a subpopulation of neurons in the dorsal raphe nucleus, including neurons projecting to the basolateral amygdaloid complex.Neuroscience. 2008 Dec 10;157:733-48.
  • IHC - Anti-Rabbit Tryptophan Hydroxylase Antibody  ABD10815
    Published customer image:Sheep antiRabbit tryptophan hydroxylase antibody used for immunostaining neuroins in rat brain by immunohistochemistry on cryostat sections.Image caption:Fos expression in TrpOH-immunostained neurons in the DRN/MnR and ChAT-immunostained neurons in the PPT/LDT. A. Illustrations of brain sections of the DRN and MnR. B. Photomicrograph illustrating an example of Fos expression in TrpOH-immunostained neurons in the DRN and MnR. Scale bars = 100 μm. C and D. Fos expression positive ratio in serotonergic neurons in the DRN, there were no difference among groups. E and F. Fos expression positive ratio in serotonergic neurons in the MnR, there were no difference among groups. G and I. Illustrations of brain sections of the PPT and LDT. H and J. Photomicrograph illustrating an example of Fos expression in ChAT-immunostained neurons in the PPT and LDT. Scale bars = 100 μm. K and L. Fos expression positive ratio in cholinergic neurons in the PPT, there were no difference among groups. M and N. Fos expression positive ratio in cholinergic neurons in the LDT, there were no difference among groups. Data are represented as mean ± SEM.From: Yu B, Cui SY, Zhang XQ, Cui XY, Li SJ, Sheng ZF, Cao Q, Huang YL, Xu YP, Lin ZG, Yang G, Song JZ, Ding H, Zhang YH. Mechanisms Underlying Footshock and Psychological Stress-Induced Abrupt Awakening From Posttraumatic "Nightmares".Int J Neuropsychopharmacol. 2015 Nov 20. pii: pyv113.
  • IHC - Anti-Rabbit Tryptophan Hydroxylase Antibody  ABD10815
    Published customer image:Sheep anti Rabbit tryptophan hydroxylase antibody used to identify neurons in rat brain by immunohistochemistry on cryostat sectionsImage caption:Low magnification photomicrographs illustrating TrpOH/c-Fos-immunostained sections from different rostrocaudal levels of the DR from a control rat. TrpOH-immunopositive neurons and dendrites can be identified by the brown/orange precipitate within subdivisions of the DR. The subdivisions of the DR analyzed are illustrated by dashed lines (adapted from a standard stereotaxic atlas of the rat brain [42]). Abbreviations: Aq, cerebral aqueduct; DRC, dorsal raphe nucleus, caudal part; DRD, dorsal raphe nucleus, dorsal part; DRI, dorsal raphe nucleus, interfascicular part; DRV, dorsal raphe nucleus, ventral part; DRVL, dorsal raphe nucleus, ventrolateral part; mlf, medial longitudinal fasciculus; xscp, decussation of the superior cerebellar peduncle. Scale bar, 500μm.From: Bouwknecht JA, Spiga F, Staub DR, Hale MW, Shekhar A, Lowry CA. Differential effects of exposure to low-light or high-light open-field on anxiety-related behaviors: relationship to c-Fos expression in serotonergic and non-serotonergic neurons in the dorsal raphe nucleus.Brain Res Bull. 2007 Apr 2;72(1):32-43.
  • IHC - Anti-Rabbit Tryptophan Hydroxylase Antibody  ABD10815
    Published customer image:Sheep anti Rabbit tryptophan hydroxylase antibody used for the demonstration of immunoreactive neurons in rat brain by immunohistochemistry on cryostat sections.Image caption:Photomicrographs illustrating the effects of exposure to the open-field in low-light, or high-light conditions compared to home cage controls, on c-Fos expression in serotonergic and non-serotonergic neurons in the mid-rostrocaudal dorsal raphe nucleus (-8.18 mm Bregma). Photomicrographs illustrate c-Fos-ir nuclei and TrpOH-ir neurons in sections from rats exposed to A) CO, B) LL, C) HL conditions in Experiment 1. Black boxes indicate regions shown at higher magnification in insets in the lower left hand corner of each panel. Arrows indicate examples of c-Fos-ir cells (blue/black nuclear staining); white arrowheads indicate c-Fos-immunonegative/TrpOH-ir neurons (brown/orange cytoplasmic staining), filled arrowheads indicate c-Fos-ir/TrpOH-ir neurons. Abbreviation: Aq, cerebral aqueduct; bv, blood vessel, characteristic of the DRVL region at this rostrocaudal level; mlf, medial longitudinal fasciculus. Scale bar, 100 μm, inset 25 μm.From: Hale MW, Hay-Schmidt A, Mikkelsen JD, Poulsen B, Bouwknecht JA, Evans AK, Stamper CE, Shekhar A, Lowry CA. Exposure to an open-field arena increases c-Fos expression in a subpopulation of neurons in the dorsal raphe nucleus, including neurons projecting to the basolateral amygdaloid complex.Neuroscience. 2008 Dec 10;157(4):733-48.
  • IHC - Anti-Rabbit Tryptophan Hydroxylase Antibody  ABD10815
    Published customer image:Sheep anti Rabbit tryptophan hydroxylase antibody used for the demonstration of immunoreactive neurons in rat brain by immunohistochemistry on cryostat sections.Image caption:Low magnification photomicrographs illustrating TrpOH/c-Fos-immunostained sections from different rostrocaudal levels of the midbrain raphe nuclei from a control rat. TrpOH-ir neurons and dendrites can be identified by the brown/orange precipitate within subdivisions of the DR and MnR. The subdivisions of the DR and MnR analyzed are illustrated by dashed lines (adapted from a standard stereotaxic atlas of the rat brain; Paxinos and Watson, 1998). Abbreviations: Aq, cerebral aqueduct; DRC, dorsal raphe nucleus, caudal part; DRD, dorsal raphe nucleus, dorsal part; DRI, dorsal raphe nucleus, interfascicular part; DRV, dorsal raphe nucleus, ventral part; DRVL, dorsal raphe nucleus, ventrolateral part; mlf, medial longitudinal fasciculus; MnR, median raphe nucleus; xscp, decussation of the superior cerebellar peduncle. Scale bar, 500 μm.From: Hale MW, Hay-Schmidt A, Mikkelsen JD, Poulsen B, Bouwknecht JA, Evans AK, Stamper CE, Shekhar A, Lowry CA. Exposure to an open-field arena increases c-Fos expression in a subpopulation of neurons in the dorsal raphe nucleus, including neurons projecting to the basolateral amygdaloid complex.Neuroscience. 2008 Dec 10;157(4):733-48.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-F
Primary Accession P17290
Reactivity Rabbit
Host Sheep
Clonality Polyclonal
Isotype Polyclonal IgG
Calculated MW 51118 Da
Additional Information
Other Species Rat,H
Purification Purified Ig prepared by affinity chromatography
Immunogen Recombinant rabbit tryptophan hydroxylase, isolated as inclusion bodies from E. coli and purified by preparative SDS-PAGE.
Shelf Life 18 months from date of despatch.
Gene ID 100009100;100101558
Other Names Tryptophan 5-hydroxylase 1, 1.14.16.4, Tryptophan 5-monooxygenase 1, TPH1, TPH
Target/Specificity Sheep anti-Rabbit tryptophan hydroxylase antibody recognises tryptophan hydroxylase, also known as Tryptophan 5-hydroxylase 1 or Tryptophan 5-monooxygenase 1. Tryptophan hydroxylase is a 444 amino acid ~55 kDa enzyme involved in the synthesis of the neurotransmitter serotonin.
Preservative & Stabilisers 0.09% Sodium Azide (NaN3)
Storage Store at +4℃ or at -20 ℃.
PrecautionsAnti-Rabbit Tryptophan Hydroxylase Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
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References

1. Lowry, A. et al (2000) Corticotropin-Releasing Factor Increases In Vitro Firing Rates of Serotonergic Neurons in the Rat Dorsal Raphe Nucleus: Evidence for Activation of a Topographically Organized Mesolimbocortical Serotonergic System.
J. Neurosci 20: 7728 - 7736 2. Federici, L.M. et al. (2015) Hypothalamic orexin's role in exacerbated cutaneous vasodilation responses to an anxiogenic stimulus in a surgical menopause model
Psychoneuroendocrinology. 18 Dec. [Epub ahead of print] 3. Johnson, P.L. et al. (2005) Acute hypercarbic gas exposure reveals functionally distinct subpopulations of serotonergic neurons in rats.
J Psychopharmacol. 19 (4): 327-41. 4. Sajdyk, T.J. et al. (2008) Neuropeptide Y in the amygdala induces long-term resilience to stress-induced reductions in social responses but not hypothalamic-adrenal-pituitary axis activity or hyperthermia.
J Neurosci. 28 (4): 893-903. 5. Johnson, P. et al. (2008) Disruption of GABAergic tone in the dorsomedial hypothalamus attenuates responses in a subset of serotonergic neurons in the dorsal raphe nucleus following lactate-induced panic.
J Psychopharmacol. 22 (6): 642-52. 6. Yu, B. et al. (2015) Mechanisms Underlying Footshock and Psychological Stress-Induced Abrupt Awakening From Posttraumatic"Nightmares".
Int J Neuropsychopharmacol. pii: pyv113. 7. Abrams, J.K. et al. (2005) Serotonergic systems associated with arousal and vigilance behaviors following administration of anxiogenic drugs.
Neuroscience. 133 (4): 983-97. 8. Staub, D.R. et al. (2005) Urocortin 2 increases c-Fos expression in topographically organized subpopulations of serotonergic neurons in the rat dorsal raphe nucleus.
Brain Res. 1044 (2): 176-89. 9. Cui, S.Y. et al. (2011) Diltiazem potentiates pentobarbital-induced hypnosis via 5-HT1A and 5-HT2A/2C receptors: role for dorsal raphe nucleus.
Pharmacol Biochem Behav. 99 (4): 566-72. 10. Bouwknecht, J.A. et al. (2007) Differential effects of exposure to low-light or high-light open-field on anxiety-related behaviors: relationship to c-Fos expression in serotonergic and non-serotonergic neurons in the dorsal raphe nucleus.
Brain Res Bull. 72 (1): 32-43. 11. Gardner, K.L. et al. (2005) Early life experience alters behavior during social defeat: focus on serotonergic systems.
Neuroscience. 136 (1): 181-91. 12. Hale, M.W. et al. (2008) Exposure to an open-field arena increases c-Fos expression in a subpopulation of neurons in the dorsal raphe nucleus, including neurons projecting to the basolateral amygdaloid complex.
Neuroscience. 157 (4): 733-48. 13. Spiga, F. et al. (2006) Injections of urocortin 1 into the basolateral amygdala induce anxiety-like behavior and c-Fos expression in brainstem serotonergic neurons.
Neuroscience. 138 (4): 1265-76. 14. Rook, G. et al. (2003) Treatment of conditions of the central nervous system using mycobacteria
U.S. Patent Publication US20030170275 A1 15. Staub, D.R. et al. (2006) Evidence supporting a role for corticotropin-releasing factor type 2 (CRF2) receptors in the regulation of subpopulations of serotonergic neurons.
Brain Res. 1070 (1): 77-89. 16. Lowry, C.A. et al. (2007) Identification of an immune-responsive mesolimbocortical serotonergic system: potential role in regulation of emotional behavior.
Neuroscience. 146 (2): 756-72.

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