|Application ||WB, IHC-F|
|Calculated MW||51118 Da|
|Purification||Purified Ig prepared by affinity chromatography|
|Immunogen||Recombinant rabbit tryptophan hydroxylase, isolated as inclusion bodies from E. coli and purified by preparative SDS-PAGE.|
|Shelf Life||18 months from date of despatch.|
|Other Names||Tryptophan 5-hydroxylase 1, 188.8.131.52, Tryptophan 5-monooxygenase 1, TPH1, TPH|
|Target/Specificity||Sheep anti-Rabbit tryptophan hydroxylase antibody recognises tryptophan hydroxylase, also known as Tryptophan 5-hydroxylase 1 or Tryptophan 5-monooxygenase 1. Tryptophan hydroxylase is a 444 amino acid ~55 kDa enzyme involved in the synthesis of the neurotransmitter serotonin.|
|Preservative & Stabilisers||0.09% Sodium Azide (NaN3)|
|Storage||Store at +4℃ or at -20 ℃.|
|Precautions||Anti-Rabbit Tryptophan Hydroxylase Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
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Provided below are standard protocols that you may find useful for product applications.
1. Lowry, A. et al (2000) Corticotropin-Releasing Factor Increases In Vitro Firing Rates of Serotonergic Neurons in the Rat Dorsal Raphe Nucleus: Evidence for Activation of a Topographically Organized Mesolimbocortical Serotonergic System.J. Neurosci 20: 7728 - 7736 2. Federici, L.M. et al. (2015) Hypothalamic orexin's role in exacerbated cutaneous vasodilation responses to an anxiogenic stimulus in a surgical menopause modelPsychoneuroendocrinology. 18 Dec. [Epub ahead of print] 3. Johnson, P.L. et al. (2005) Acute hypercarbic gas exposure reveals functionally distinct subpopulations of serotonergic neurons in rats.J Psychopharmacol. 19 (4): 327-41. 4. Sajdyk, T.J. et al. (2008) Neuropeptide Y in the amygdala induces long-term resilience to stress-induced reductions in social responses but not hypothalamic-adrenal-pituitary axis activity or hyperthermia.J Neurosci. 28 (4): 893-903. 5. Johnson, P. et al. (2008) Disruption of GABAergic tone in the dorsomedial hypothalamus attenuates responses in a subset of serotonergic neurons in the dorsal raphe nucleus following lactate-induced panic.J Psychopharmacol. 22 (6): 642-52. 6. Yu, B. et al. (2015) Mechanisms Underlying Footshock and Psychological Stress-Induced Abrupt Awakening From Posttraumatic"Nightmares".Int J Neuropsychopharmacol. pii: pyv113. 7. Abrams, J.K. et al. (2005) Serotonergic systems associated with arousal and vigilance behaviors following administration of anxiogenic drugs.Neuroscience. 133 (4): 983-97. 8. Staub, D.R. et al. (2005) Urocortin 2 increases c-Fos expression in topographically organized subpopulations of serotonergic neurons in the rat dorsal raphe nucleus.Brain Res. 1044 (2): 176-89. 9. Cui, S.Y. et al. (2011) Diltiazem potentiates pentobarbital-induced hypnosis via 5-HT1A and 5-HT2A/2C receptors: role for dorsal raphe nucleus.Pharmacol Biochem Behav. 99 (4): 566-72. 10. Bouwknecht, J.A. et al. (2007) Differential effects of exposure to low-light or high-light open-field on anxiety-related behaviors: relationship to c-Fos expression in serotonergic and non-serotonergic neurons in the dorsal raphe nucleus.Brain Res Bull. 72 (1): 32-43. 11. Gardner, K.L. et al. (2005) Early life experience alters behavior during social defeat: focus on serotonergic systems.Neuroscience. 136 (1): 181-91. 12. Hale, M.W. et al. (2008) Exposure to an open-field arena increases c-Fos expression in a subpopulation of neurons in the dorsal raphe nucleus, including neurons projecting to the basolateral amygdaloid complex.Neuroscience. 157 (4): 733-48. 13. Spiga, F. et al. (2006) Injections of urocortin 1 into the basolateral amygdala induce anxiety-like behavior and c-Fos expression in brainstem serotonergic neurons.Neuroscience. 138 (4): 1265-76. 14. Rook, G. et al. (2003) Treatment of conditions of the central nervous system using mycobacteria U.S. Patent Publication US20030170275 A1 15. Staub, D.R. et al. (2006) Evidence supporting a role for corticotropin-releasing factor type 2 (CRF2) receptors in the regulation of subpopulations of serotonergic neurons.Brain Res. 1070 (1): 77-89. 16. Lowry, C.A. et al. (2007) Identification of an immune-responsive mesolimbocortical serotonergic system: potential role in regulation of emotional behavior.Neuroscience. 146 (2): 756-72.
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