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>   home   >   Products   >   Primary Antibodies   >   Stem Cells   >   Anti-Human TNF alpha Antibody    

Anti-Human TNF alpha Antibody

Sheep Anti-Human Polyclonal Antibody

Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P01375
Reactivity Human
Host Sheep
Clonality Polyclonal
Isotype Polyclonal IgG
Calculated MW 25644 Da
Additional Information
Purification Ig fraction prepared by ammonium sulphate precipitation
Immunogen Recombinant Human TNF alpha from an Escherichia coli expression system.
Shelf Life 18 months from date of despatch.
Gene ID 7124
Other Names Tumor necrosis factor, Cachectin, TNF-alpha, Tumor necrosis factor ligand superfamily member 2, TNF-a, Tumor necrosis factor, membrane form, N-terminal fragment, NTF, Intracellular domain 1, ICD1, Intracellular domain 2, ICD2, C-domain 1, C-domain 2, Tumor necrosis factor, soluble form, TNF, TNFA, TNFSF2
Target/Specificity Intended UseTarget SpeciesHumanProduct FormIg fraction - liquid ReconstitutionPreparationIg fraction prepared by ammonium sulphate precipitationPreservative Stabilisers0.09% Sodium Azide (NaN3)ImmunogenRecombinant Human TNF alpha from an Escherichia coli expression system.PurityApprox. Protein ConcentrationsIgG concentration 5.0 mg/mlReagents In The KitPreparing The AntibodyTest PrincipleBuffer SolutionPhosphate buffered salineStorageStore at +4oC or at -20oC if preferred.Storage in frost-free freezers is not recommended.This product should be stored undiluted.Avoid repeated freezing and thawing as this may denature the antibody.Should this product contain a precipitate we recommend microcentrifugation before use.Shelf Life18 months from date of despatch.GO TermsGO:0042346positive regulation of NF-kappaB import into nucleus;GO:0000185activation of MAPKKK activity;GO:0050995negative regulation of lipid catabolic process;GO:0070374positive regulation of ERK1 and ERK2 cascade;GO:0030730sequestering of triglyceride;GO:0010888negative regulation of lipid storage;GO:0043123positive regulation of I-kappaB kinase/NF-kappaB cascade;GO:0034116positive regulation of heterotypic cell-cell adhesion;GO:0005164tumor necrosis factor receptor binding;GO:0051044positive regulation of membrane protein ectodomain proteolysis;GO:0032582negative regulation of gene-specific transcription;GO:0032800receptor biosynthetic process;GO:0005125cytokine activity;GO:0002740negative regulation of cytokine secretion involved in immune response;GO:0000187activation of MAPK activity;GO:0033209tumor necrosis factor-mediated signaling pathway;GO:0050901leukocyte tethering or rolling;GO:0033138positive regulation of peptidyl-serine phosphorylation;GO:0045071negative regulation of viral genome replication;GO:0045080positive regulation of chemokine biosynthetic process;GO:0009615response to virus;GO:0060559positive regulation of calcidiol 1-monooxygenase activity;GO:0010843promoter binding;GO:0032722positive regulation of chemokine production;GO:0031622positive regulation of fever generation;GO:0051384response to glucocorticoid stimulus;GO:0050715positive regulation of cytokine secretion;GO:0009897external side of plasma membrane;GO:0045672positive regulation of osteoclast differentiation;GO:0016563transcription activator activity;GO:0060555induction of necroptosis by extracellular signals;GO:0006919activation of caspase activity;GO:0051092positive regulation of NF-kappaB transcription factor activity;GO:0001891phagocytic cup;GO:0005615extracellular space;GO:0048661positive regulation of smooth muscle cell proliferation;GO:0043193positive regulation of gene-specific transcription;GO:0006916anti-apoptosis;GO:0055037recycling endosome;GO:0050796regulation of insulin secretion;GO:0043243positive regulation of protein complex disassembly;GO:0042802identical protein binding;GO:0000060protein import into nucleus, translocation;GO:0032715negative regulation of interleukin-6 production;GO:0005887integral to plasma membrane;GO:0006927transformed cell apoptosis;GO:0061048negative regulation of branching involved in lung morphogenesis;GO:0009651response to salt stress;GO:0045429positive regulation of nitric oxide biosynthetic process;GO:0045599negative regulation of fat cell differentiation;GO:0070265necrotic cell death;GO:0060557positive regulation of vitamin D biosynthetic process;GO:0016564transcription repressor activity;GO:0045121membrane raft;GO:0002439chronic inflammatory response to antigenic stimulus;GO:0031334positive regulation of protein complex assembly;UniProtP01375;Entrez GeneTNF;AcknowledgementsRegulatoryFor research purposes only
Preservative & Stabilisers 0.09% Sodium Azide (NaN3)
Storage Store at +4℃ or at -20 ℃.
PrecautionsAnti-Human TNF alpha Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
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Cat# ABD10817
(40 western blots)
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