|Calculated MW||25644 Da|
|Purification||Ig fraction prepared by ammonium sulphate precipitation|
|Immunogen||Recombinant Human TNF alpha from an Escherichia coli expression system.|
|Shelf Life||18 months from date of despatch.|
|Other Names||Tumor necrosis factor, Cachectin, TNF-alpha, Tumor necrosis factor ligand superfamily member 2, TNF-a, Tumor necrosis factor, membrane form, N-terminal fragment, NTF, Intracellular domain 1, ICD1, Intracellular domain 2, ICD2, C-domain 1, C-domain 2, Tumor necrosis factor, soluble form, TNF, TNFA, TNFSF2|
|Target/Specificity||Intended UseTarget SpeciesHumanProduct FormIg fraction - liquid ReconstitutionPreparationIg fraction prepared by ammonium sulphate precipitationPreservative Stabilisers0.09% Sodium Azide (NaN3)ImmunogenRecombinant Human TNF alpha from an Escherichia coli expression system.PurityApprox. Protein ConcentrationsIgG concentration 5.0 mg/mlReagents In The KitPreparing The AntibodyTest PrincipleBuffer SolutionPhosphate buffered salineStorageStore at +4oC or at -20oC if preferred.Storage in frost-free freezers is not recommended.This product should be stored undiluted.Avoid repeated freezing and thawing as this may denature the antibody.Should this product contain a precipitate we recommend microcentrifugation before use.Shelf Life18 months from date of despatch.GO TermsGO:0042346positive regulation of NF-kappaB import into nucleus;GO:0000185activation of MAPKKK activity;GO:0050995negative regulation of lipid catabolic process;GO:0070374positive regulation of ERK1 and ERK2 cascade;GO:0030730sequestering of triglyceride;GO:0010888negative regulation of lipid storage;GO:0043123positive regulation of I-kappaB kinase/NF-kappaB cascade;GO:0034116positive regulation of heterotypic cell-cell adhesion;GO:0005164tumor necrosis factor receptor binding;GO:0051044positive regulation of membrane protein ectodomain proteolysis;GO:0032582negative regulation of gene-specific transcription;GO:0032800receptor biosynthetic process;GO:0005125cytokine activity;GO:0002740negative regulation of cytokine secretion involved in immune response;GO:0000187activation of MAPK activity;GO:0033209tumor necrosis factor-mediated signaling pathway;GO:0050901leukocyte tethering or rolling;GO:0033138positive regulation of peptidyl-serine phosphorylation;GO:0045071negative regulation of viral genome replication;GO:0045080positive regulation of chemokine biosynthetic process;GO:0009615response to virus;GO:0060559positive regulation of calcidiol 1-monooxygenase activity;GO:0010843promoter binding;GO:0032722positive regulation of chemokine production;GO:0031622positive regulation of fever generation;GO:0051384response to glucocorticoid stimulus;GO:0050715positive regulation of cytokine secretion;GO:0009897external side of plasma membrane;GO:0045672positive regulation of osteoclast differentiation;GO:0016563transcription activator activity;GO:0060555induction of necroptosis by extracellular signals;GO:0006919activation of caspase activity;GO:0051092positive regulation of NF-kappaB transcription factor activity;GO:0001891phagocytic cup;GO:0005615extracellular space;GO:0048661positive regulation of smooth muscle cell proliferation;GO:0043193positive regulation of gene-specific transcription;GO:0006916anti-apoptosis;GO:0055037recycling endosome;GO:0050796regulation of insulin secretion;GO:0043243positive regulation of protein complex disassembly;GO:0042802identical protein binding;GO:0000060protein import into nucleus, translocation;GO:0032715negative regulation of interleukin-6 production;GO:0005887integral to plasma membrane;GO:0006927transformed cell apoptosis;GO:0061048negative regulation of branching involved in lung morphogenesis;GO:0009651response to salt stress;GO:0045429positive regulation of nitric oxide biosynthetic process;GO:0045599negative regulation of fat cell differentiation;GO:0070265necrotic cell death;GO:0060557positive regulation of vitamin D biosynthetic process;GO:0016564transcription repressor activity;GO:0045121membrane raft;GO:0002439chronic inflammatory response to antigenic stimulus;GO:0031334positive regulation of protein complex assembly;UniProtP01375;Entrez GeneTNF;AcknowledgementsRegulatoryFor research purposes only|
|Preservative & Stabilisers||0.09% Sodium Azide (NaN3)|
|Storage||Store at +4℃ or at -20 ℃.|
|Precautions||Anti-Human TNF alpha Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
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