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Anti-Vancomycin Antibody

     
  • E - Anti-Vancomycin Antibody  ABD10822
    Published customer image:Rabbit anti vancomycin antibody used to detect vancomycin-BSA conjugate by western blottingImage caption:Hapten evaluation. a Ponceau S staining indicates protein presence in the coupled haptens. b Immunoblotting indicates corresponding antibiotic presence in both coupled haptens specifically.From: ELISA-based detection of gentamicin and vancomycin in protein-containing samples. Odekerken, J.C.E. et al.SpringerPlus 2015, 4:614
  • E - Anti-Vancomycin Antibody  ABD10822
    Published customer image:Rabbit anti vancomycin antibody used to detect vancomycin-BSA conjugate by ELISAImage caption:Schematic representation of the antibiotic ELISA procedure and detection. The antibiotic-BSA hapten is coated to a microtiter plate. An antibody directed against the antibiotic compound can interact with either the antibiotic in the sample or the antibiotic-hapten coated to the microtiter plate. After washing only the microtiter plate-bound antibodies remain present and can be detected by HRP-conjugated secondary antibodies in combination with TMB (colorimetric detection at 450 nm). A high concentration of antibiotics in a sample will lead to more interaction with an antibiotic-specific antibody in solution and thus result in less bound antibodies to the microtiter plate leading to a low signal when detected at 450 nm. This indicates that the measured signal is inversely correlated with the concentration of antibiotic in the measured sample. Samples with a known concentration of antibiotics can be used for a calibration curve; regression from this curve will allow calculation of the antibiotic concentration in unknown samples.From: ELISA-based detection of gentamicin and vancomycin in protein-containing samples. Odekerken, J.C.E. et al.SpringerPlus 2015, 4:614
  • E - Anti-Vancomycin Antibody  ABD10822
    Published customer image:Rabbit anti vancomycin antibody used to detect vancomycin-BSA conjugate by ELISAImage caption:Coating optimization. a The optimal concentration of the gentamicin-BSA hapten was based on the pattern of the calibration curve, which indicated a concentration dependant absorbance. b The optimal concentration of the vancomycin-BSA hapten was determined on the relation between coating concentration and absorbance level.From: ELISA-based detection of gentamicin and vancomycin in protein-containing samples. Odekerken, J.C.E. et al.SpringerPlus 2015, 4:614
  • E - Anti-Vancomycin Antibody  ABD10822
    Published customer image:Rabbit anti vancomycin antibody used to detect vancomycin-BSA conjugate by ELISAImage caption:Specificity and sensitivity of the antibiotic ELISAs. a The influence of vancomycin on the gentamicin ELISA. b The influence of gentamicin on the vancomycin ELISA. c The validation of the calibration curve of the gentamicin ELISA. Lines indicate upper and lower 10 % range of the calibration curve. d The validation of the calibration curve of the vancomycin ELISA. Lines indicate upper and lower 10 % range of the calibration curve. Error bars indicate standard deviation.From: ELISA-based detection of gentamicin and vancomycin in protein-containing samples. Odekerken, J.C.E. et al.SpringerPlus 2015, 4:614
  • E - Anti-Vancomycin Antibody  ABD10822
    Published customer image:Rabbit anti vancomycin antibody used to detect vancomycin-BSA conjugate by ELISAImage caption:ELISA for gentamicin and vancomycin. A combined hapten of both gentamicin and vancomycin was used to coat wells for the detection of either gentamicin (black circles) or vancomycin (black squares) in a calibrations series of the respective antibiotics. Error bars indicate standard deviation.From: ELISA-based detection of gentamicin and vancomycin in protein-containing samples. Odekerken, J.C.E. et al.SpringerPlus 2015, 4:614
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, E
Host Rabbit
Clonality Polyclonal
Isotype Polyclonal IgG
Additional Information
Purification Purified IgG prepared by affinity chromatography on Protein G
Immunogen Vancomycin conjugated to Keyhole limpet Haemocyanin plus thyroglobulin.
Shelf Life 18 months from date of despatch.
Target/Specificity Rabbit anti-Vancomycin antibody recognizes vancomycin, a glycopeptide antibiotic derived from the actinobacterium Amycolatopsis orientalis that acts by inhibiting cell wall biosynthesis and altering the permeability of the bacterial cell membrane (Cheung & DiPiro 1986) . Vancomycin is regarded as an antibiotic of last resort as in high doses it can be toxic to the ears (Lackner 1984) and kidneys (Hanrahanet al.2015), whilst in low doses hypersensitivity reactions may occur (Blumenthalet al.2015).Rabbit anti-Vancomycin antibody has been used for the development of a competitive ELISA for the detection of vancomycin in protein containing samples (Oderkerken et al. 2015)
Preservative & Stabilisers 0.09% Sodium Azide (NaN3)
Storage Store at +4℃ or at -20 ℃.
PrecautionsAnti-Vancomycin Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
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References

1. Odekerken, J.C.E. et al. (2015) ELISA-based detection of gentamicin and vancomycin in protein-containing samples
SpringerPlus. 4 (1): 614.

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Cat# ABD10822
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