|Application ||WB, IHC-F, IP|
|Calculated MW||133108 Da|
|Purification||Purified IgG prepared by affinity chromatography on Protein A|
|Immunogen||Recombinant XPG protein produced in baculovirus (185 kD).|
|Shelf Life||18 months from date of despatch.|
|Other Names||DNA repair protein complementing XP-G cells, 3.1.-.-, DNA excision repair protein ERCC-5, Xeroderma pigmentosum group G-complementing protein, ERCC5, ERCM2, XPG, XPGC|
|Target/Specificity||ABD10833 is specific for the Xeroderma Pigmentosum protein G, a DNA endonuclease involved in the repair of DNA damage. Patients with Xeroderma Pigmentosum have defective ERCC5 genes resulting in very low levels of the endonuclease. Such patients are extremely photosensitive, presenting with erythema and blistering amongst other abnormalities. Due to the severe deficiency in nucleotide excision repair, the skin fibroblasts of patients are exquisitively sensitive to UV leaving them vulnerable to developing skin cancer.|
|Preservative & Stabilisers||0.09% Sodium Azide (NaN3)|
|Storage||Store at +4℃ or at -20 ℃.|
|Precautions||Anti-XPG Antibody, clone 8H7 is for research use only and not for use in diagnostic or therapeutic procedures.|
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Provided below are standard protocols that you may find useful for product applications.
1. Evans, E. et al. (1997) Open complex formation around a lesion during nucleotide excision repair provides a structure for cleavage by human XPG protein.EMBO J. 16:625-38. 2. O'Donovan, A. et al. (1994) Isolation of active recombinant XPG protein, a human DNA repair endonuclease.J Biol Chem. 269:15965-8. 3. Nouspikel, T. et al. (1997) A common mutational pattern in Cockayne syndrome patients from xeroderma pigmentosum group G: implications for a second XPG function.Proc Natl Acad Sci USA. 94:3116-21. 4. Caonstaintinou, A. et al. (1999) Conserved residues of human XPG protein important for nuclease activity and function in nucleotide excision repair.J Biol Chem.274:5637-48. 5. Araujo, S. J., Nigg, E. A. and Wood, R. D. (2001) Strong functional interactions of TFIIH with XPC and XPG in human DNA nucleotide excision repair, without a preassembled repairosome.Mol Cell Biol. 21:2281-91.
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