|Calculated MW||63225 Da|
|Purification||Antisera to LNK (NT) were raised by repeated immunisation of Goat with highly purified antigen.|
|Immunogen||Peptide sequence NGPALQPSSPC from LNK amino-terminus.|
|Shelf Life||18 months from date of despatch.|
|Other Names||SH2B adapter protein 3, Lymphocyte adapter protein, Lymphocyte-specific adapter protein Lnk, Signal transduction protein Lnk, SH2B3, LNK|
|Target/Specificity||Goat anti-Human LNK antibodyrecognizes human SH2B adapter protein 3, also known as Lymphocyte-specific adapter protein Lnk or Lymphocyte adapter protein. LNK is a 575 amino acid SH2B adapteer protein containing a single PH domain and a single SH2 domain. Goat anti-Human LNK antibody binds to an epitope within the N-terminal region of LNK, which regulates cytokine receptor-mediated and growth factor pathways, and plays an essential role in B cell lymphopoiesis.LNK mRNA is preferentially expressed in lymph node and spleen lymphocytes and the expression of LNK in vascular endothelial cells (ECs), increases in response to TNFα. LNK acts as a negative regulator of the pro-inflammatory molecules VCAM-1 and E-selectin induced by TNFα, modulation of P13-kinase and MAPK ERK1/2 activity. LNK is also involved in the negative regulation of Thrombopoietin-mediated cell proliferation and endomitosis in hematopoietic cell lines.|
|Preservative & Stabilisers||0.02% Sodium Azide; 0.5% Bovine Serum Albumin;|
|Storage||Store at +4℃ or at -20 ℃.|
|Precautions||Anti-Human Lnk (N-Terminal) Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
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Provided below are standard protocols that you may find useful for product applications.
1. Fitau, J. et al. (2006) The adaptor molecule LNK negatively regulates TNFα-dependent VCAM-1 expression in endothelial cells through inhibition of the ERK1 and 2 pathways. J. Biol. Chem. 281: 20148-59 2. Devallière, J. et al. (2012) LNK (SH2B3) is a key regulator of integrin signaling in endothelial cells and targets a-parvin to control cell adhesion and migration.FASEB J. 26: 2592-606. 3. Gery, S. et al. (2007) Adaptor protein Lnk negatively regulates the mutant MPL, MPLW515L associated with myeloproliferative disorders.Blood. 110: 3360-4. 4. Gery, S. et al.M (2009) Lnk inhibits myeloproliferative disorder-associated JAK2 mutant, JAK2V617F.J Leukoc Biol. 85: 957-65. 5. Wan, M. et al. (2006) TNF-a Induces Lnk Expression Through PI3K-Dependent Signaling Pathway in Human Umbilical Vein Endothelial CellsJ Surg Res. 136: 53-7. 6. Fitau, J. et al. (2006) The adaptor molecule Lnk negatively regulates tumor necrosis factor-alpha-dependent VCAM-1 expression in endothelial cells through inhibition of the ERK1 and -2 pathways.J Biol Chem. 281: 20148-59. 7. Wan, M. et al. (2007) Eicosapentaenoic acid inhibits TNF-alpha-induced Lnk expression in human umbilical vein endothelial cells: involvement of the PI3K/Akt pathway.J Nutr Biochem. 18: 17-22. 8. Chatelais, M. et al. (2011) Gene transfer of the adaptor Lnk (SH2B3) prevents porcine endothelial cell activation and apoptosis: implication for xenograft's cytoprotection.Xenotransplantation. 18: 108-20. 9. Devallière, J. et al. (2012) LNK (SH2B3) is a key regulator of integrin signaling in endothelial cells and targets a-parvin to control cell adhesion and migration.FASEB J. 26: 2592-606. 10. Fitau, J. et al. (2005) The adaptor protein Lnk modulates endothelial cell activation.Nephrol Ther. 1: 228-33.1. Tong, W. and Lodish, H.F. (2004) Lnk inhibits Tpo-mpl signaling and Tpo-mediated megakaryocytopoiesis.J. Exp. Med. 200: 569-580.
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