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Anti-BAP31 Antibody

Rabbit Anti-Human Polyclonal Antibody

  • WB - Anti-BAP31 Antibody  ABD10874
    Western blot analysis of rat lung lysate probed with Rabbit anti Human BAP31 at 0.5(A), 1(B) and 2(C) ug/ml
  • IHC-P - Anti-BAP31 Antibody  ABD10874
    Formilin fixed, paraffin embedded rat lung stained with Rabbit anti Human BAP31 following heat mediated antigen retrieval using sodium citrate buffer (pH6.0)
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P51572
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Polyclonal IgG
Calculated MW 27992 Da
Additional Information
Other Species M,Rat
Purification Antisera to human BAP31 were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG prepared by affinity chromatography.
Immunogen A 17 amino acid peptide located in the internal region of human BAP31.
Shelf Life 18 months from date of despatch.
Gene ID 10134
Other Names B-cell receptor-associated protein 31, BCR-associated protein 31, Bap31, 6C6-AG tumor-associated antigen, Protein CDM, p28, BCAP31, BAP31, DXS1357E
Target/Specificity Rabbit anti-human BAP31 recognizes an epitope within the internal region of BAP31 (B cell receptor-associated protein 31), a ~28 kDa conserved type III integral membrane protein of the endoplasmic reticulum (ER), containing two caspase cleavage sites within its cytosolic domain.BAP31 appears to be a multi-functional ER-associated transport protein which was originally identified through its association with membrane IgD in B lymphocytes. Several proteins have since been shown to associate with BAP31, including the Beta2 integrin receptor CD11b/CD18, and nonmuscle gamma-actin and myosin B heavy chain. BAP31 has become a focus area for apoptosis studies due to its association with Bcl-2 and cleavage by initiator caspases including caspase 1 and 8. Cleavage of BAP31 generates a membrane-integrated p20 fragment which is itself a potent inducer of apoptosis when expressed ectopically.
Preservative & Stabilisers 0.02% Sodium Azide (NaN3)
Storage Store at +4℃ or at -20 ℃.
PrecautionsAnti-BAP31 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
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1. Kim, K-M. et al. (1994) Two new proteins preferentially associated with membrane immunoglobulin D.
EMBO J. 13: 3793-3800.2. Ng, F.W.H. et al. (1997) p28 Bap31, a Bcl-2/Bcl-XL- and procaspase-8-associated protein in the Endoplasmic Reticulum.
J. Cell Biol. 139: 327-338.3. Ducret, A. et al. (2003) The resident endoplasmic reticulum protein, BAP31, associates with gamma-actin and myosin B heavy chain.
Eur. J. Biochem. 270: 342-349.4. Zen, K. et al. (2004) Association of BAP31 with CD11b/CD18. Potential role in intracellular trafficking of CD11b/CD18 in neutrophils
J. Biol. Chem. 279: 44924-44930.

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Cat# ABD10874
(40 western blots)
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