|Application ||WB, IF, E|
|Calculated MW||15214 Da|
|Purification||Antisera to acetylated histone H3 were raised by repeated immunisation of rabbits with highly purified antigen.|
|Immunogen||Ovalbumin-conjugated peptide: NKSTGGAcKAPRKYCc|
|Shelf Life||18 months from date of despatch.|
|Other Names||Histone H3.3C, Histone H3.5, H3F3C|
|Target/Specificity||Rabbit anti-Histone acetylated H3Histone antibody was generated using a peptide corresponding to H3 acetylated at lysine 14 and recognizes all or most acetylated H3 isoforms.|
|Preservative & Stabilisers||0.02% Sodium Azide|
|Storage||Store at +4℃ or at -20 ℃.|
|Precautions||Anti-Histone H3 (Acetylated) Antibody (Serum) is for research use only and not for use in diagnostic or therapeutic procedures.|
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Provided below are standard protocols that you may find useful for product applications.
1. Belyaev, N. D. et al. (1996) Differential underacetylation of histones H2A, H3 and H4 on the inactive X chromosome in human female cells.Hum. Genet. 97: 573-578. 2. Tsaprouni, L.G. et al. (2011) Differential patterns of histone acetylation in inflammatory bowel diseases.J Inflamm (Lond). 8: 1. 3. Foy, R.L. et al. (2008) Role of Jade-1 in the histone acetyltransferase (HAT) HBO1 complex.J Biol Chem. 283: 28817-26. 4. Havasi, A. et al. (2013) Histone Acetyl Transferase (HAT) HBO1 and JADE1 in Epithelial Cell Regeneration.Am J Pathol. 182: 152-62. 5. Epping MT et al. (2009) UNC45A confers resistance to histone deacetylase inhibitors and retinoic acid.Mol Cancer Res. 7 (11): 1861-70.
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