|Application ||WB, IHC-P, E|
|Calculated MW||22054 Da|
|Purification||Antisera to sheep 14-3-3 beta were raised by repeated immunisations of rabbit with highly purified antigen.|
|Immunogen||Synthetic peptide corresponding to acetylated N-terminal sequence of sheep 14-3-3 beta.Peptide sequence: Ac.TMDKSELVC|
|Shelf Life||18 months from the date of despatch.|
|Other Names||14-3-3 protein beta/alpha, Protein kinase C inhibitor protein 1, KCIP-1, 14-3-3 protein beta/alpha, N-terminally processed, YWHAB|
|Target/Specificity||Rabbit anti-14-3-3 β antibody recognizes the acetylated N-terminal of 14-3-3 β. It is a member of the 14-3-3 family which consists of 30 kDa proteins that are involved in multiple protein kinase signalling pathways, regulation of cell cycle progression, cytoskeletal structure, transcription, intracellular trafficking and targeting. Protein interactions with 14-3-3 show distinct preference for it’s different isoforms and are regulated by phosphorylation of both 14-3-3 and the bound protein.14-3-3 β plays a role in the pathogenesis of systemic lupus erythematosus and is involved in negative regulation of p90 ribosomal S6 kinase activity and tuberous sclerosis tumour suppressor proteins. It also binds IGF-1 receptor, RAF1 and CDC25 phosphatases. Rabbit anti-14-3-3 β antibody may not react with recombinant proteins that are not N-acetylated.|
|Preservative & Stabilisers||0.09% Sodium Azide|
|Storage||Store at +4℃ or at -20 ℃.|
|Precautions||Anti-14-3-3 beta Antibody (Serum) is for research use only and not for use in diagnostic or therapeutic procedures.|
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Provided below are standard protocols that you may find useful for product applications.
1. Martin, H. et al. (1993) Antibodies against major brain isoforms of 14-3-3 protein. An antibody specific for the N-acetylated amino-terminus of a protein. FEBS Lett. 331: 296-303. 2. Chen, H.K. et al. (2003) Interaction of Akt-phosphorylated ataxin-1 with 14-3-3 mediates neurodegeneration in spinocerebellar ataxia type 1.Cell. 113: 457-68. 3. Wang, Z. et al. (2011) The prognostic value of 14-3-3 isoforms in vulvar squamous cell carcinoma cases: 14-3-3ß and e are independent prognostic factors for these tumors.PLoS One. 6: e24843. 4. Baxter, H.C. et al. (2002) Immunolocalisation of 14-3-3 isoforms in normal and scrapie-infected murine brain.Neuroscience. 109: 5-14. 5. Chaudhri, M. et al. (2003) Mammalian and yeast 14-3-3 isoforms form distinct patterns of dimers in vivo.Biochem Biophys Res Commun. 300: 679-85. 6. Taurino, F. et al. (2014) Short-term Type-1 diabetes differentially modulates 14-3-3 proteins in rat brain and liver.Eur J Clin Invest. 44: 350-8.1. Aitken, A. et al. (2006) 14-3-3 proteins: A historic overview. Seminars in Cancer Biology 16: 162-172.
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