|Application ||WB, IHC-P, E|
|Calculated MW||29174 Da|
|Purification||Antisera to sheep anti 14-3-3 epsilon C-terminus were raised by repeated immunisations rabbit of with highly purified antigen.|
|Immunogen||Synthetic corresponding to C-terminal sequence of sheep 14-3-3 epsilon.Peptide sequence: CGEEQNKEALQDVEDENQ|
|Shelf Life||18 months from date of despatch.|
|Other Names||14-3-3 protein epsilon, 14-3-3E, Protein kinase C inhibitor protein 1, KCIP-1, YWHAE|
|Target/Specificity||Rabbit anti-14-3-3 antiubody recognizes a C-terminal epitope of 14-3-3 epsilon, a member of the 14-3-3 family which consists of 30 kDa proteins involved in multiple protein kinase signalling pathways, regulation of cell cycle progression, cytoskeletal structure, transcription, intracellular trafficking and targeting. Protein interactions with 14-3-3 show distinct preference for its different isotypes and are regulated by phosphorylation of both 14-3-3 and the bound protein.14-3-3 epsilon is expressed in all mammals. It is deleted in small cell lung cancers disrupting the G checkpoint (Aitken, A. et al.2006). During apoptosis, cleavage of 14-3-3 protein by caspase-3 increases the affinity between bad and Bcl-x(L). When bound by ERK, 14-3-3 epsilon is thought to deactivate heat shock factor 1 in cells recovering from heat shock. It is also a potential suicide susceptibility gene.|
|Preservative & Stabilisers||0.09% Sodium Azide|
|Storage||Store at +4℃ or at -20 ℃.|
|Precautions||Anti-14-3-3 epsilon (C-Terminal) Antibody (Serum) is for research use only and not for use in diagnostic or therapeutic procedures.|
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