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Anti-14-3-3 eta Antibody (Serum)

Rabbit Anti-Sheep Polyclonal Antibody

  • IF - Anti-14-3-3 eta Antibody (Serum) ABD10883
    Published customer image: The 14-3-3η protein accumulates at the metaphase II spindle of the mouse egg matured in vivo and in vitro. Cells were fixed, permeabilized and immunolabeled for confocal double immunofluorescence using one of two primary antibodies against the 14-3-3η protein (red), an antibody to a-tubulin (green) and counterstained with Hoechst 33342 (blue) to visualize DNA. (A-D) A representative in vivo-matured and ovulated egg labeled with a goat antibody that recognizes the C-terminal end of the 14-3-3η protein (C). (E-H) A representative in vitro-matured egg cell that was held in prophase I arrest for 24 hours, released from the arrest and examined at 13 hours with a rabbit antibody recognizing the N-terminal end of the 14-3-3? protein (G). PB, First Polar Body. The merged image is an overlay of immunofluorescence images from the three channels. Scale bars represent 10 µm.From: De S, Kline D. Evidence for the requirement of 14-3-3η (YWHAH) in meiotic spindle assembly during mouse oocyte maturation. BMC Dev Biol. 2013 Apr 1;13:10.
  • IHC - Anti-14-3-3 eta Antibody (Serum) ABD10883
    Published customer image:Representative immunohistochemistry images of 14-3-3 η in the different stages of follicular development in ovarian sections. (A) Primordial follicle. (B) Primary follicle. (C) Secondary follicle. (D) Early antral follicle. (E) Graafian (advanced antral) follicle. (F) Corpus luteum. White arrows indicate the primordial or primary follicles in (A and B). Note the weaker staining in mural granulosa cells in secondary follicles (C, green arrows) and the more intense staining in cells lining the antral cavity (E, red arrows). The scale bars represent 10 µm (A-C) or 100 µm (D-F).From: De S, Marcinkiewicz JL, Vijayaraghavan S, Kline D. Expression of 14-3-3 protein isoforms in mouse oocytes, eggs and ovarian follicular development. BMC Res Notes. 2012 Jan 23;5:57.
  • IF - Anti-14-3-3 eta Antibody (Serum) ABD10883
    Published customer image:Representative immunocytochemistry images of 14-3-3 protein isoforms along and in the zonae pellucidae of cumulus-free oocytes isolated from ovaries of adult mice. The zona-intact cells were fixed in paraformaldehyde but not treated with detergent (see Methods). Paired images of an oocyte (left image is brightfield and right is immunofluorescence) indicate staining along the zona and/or the cell membrane for all isoforms except 14-3-3ζ. Note: the intent of this part of the study was not to examine the intracellular distribution of the isoforms (see Figure 3 for those experiments) since the cells were not permeabilized to permit complete antibody penetration; however, cells may be partially permeabilized by fixation accounting for the detection of intracellular proteins in some cells. (A,B) 14-3-3 ß. (C,D) 14-3-3 ?. (E,F) 14-3-3 e. (G,H) 14-3-3 ?. (I,J) 14-3-3 ?. (K,L) 14-3-3 t. (M,N) 14-3-3s. (O,P) Control image without primary antibody addition.From: De S, Marcinkiewicz JL, Vijayaraghavan S, Kline D. Expression of 14-3-3 protein isoforms in mouse oocytes, eggs and ovarian follicular development. BMC Res Notes. 2012 Jan 23;5:57.
  • IF - Anti-14-3-3 eta Antibody (Serum) ABD10883
    Published customer image:Representative immunofluorescence images of 14-3-3 isoforms in oocytes and eggs isolated from adult mice. (A, B) 14-3-3β. (C, D) 14-3-3γ. (E, F) 14-3-3ε. (G, H) 14-3-3ζ. (I, J) 14-3-3 τ. (K, L) 14-3-3σ. (M, N) 14-3-3η. Confocal sections with regions of red fluorescence indicating the corresponding isoforms studied (see Methods). The inset in N shows the same egg labeled blue with Hoechst DNA stain (non-confocal image) and confirms that the darker areas in this region of the larger image are condensed metaphase II chromosomes. Control cells were included for each isoform experiment and were imaged using the same confocal settings. Representative control oocytes and eggs are shown in bright-field (O) and fluorescence (P). 14-3-3? accumulates, in part, in the meiotic spindle in eggs as shown by simultaneous labeling with 14-3-3? (Q) and tubulin (R) antibodies. These sequential scans are merged (14-3-3? + tubulin) in (S). The scale bars represent 10 µm for all images.From: De S, Marcinkiewicz JL, Vijayaraghavan S, Kline D. Expression of 14-3-3 protein isoforms in mouse oocytes, eggs and ovarian follicular development. BMC Res Notes. 2012 Jan 23;5:57.
  • WB - Anti-14-3-3 eta Antibody (Serum) ABD10883
    Western blots of 14-3-3 isoforms in HEK293 cell extracts using AbD Serotec antibodies.Lane 1 = anti-gamma 14-3-3 Lane 2 = anti-zeta 14-3-3 Lane 3 = anti-C-epsilon-14-3-3 Lane 4 = anti-N-epsilon-14-3-3 Lane 5 = anti-eta 14-3-3. The 14-3-3 isoforms run at 30 kDa except epsilon 14-3-3 which migrates at 33 kDa
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q04917
Reactivity Sheep
Host Rabbit
Clonality Polyclonal
Isotype Polyclonal IgG
Calculated MW 28219 Da
Additional Information
Other Species M,H,B,C,Rb,Rat
Purification Antisera to sheep 14-3-3 eta were raised by repeated immunisations of rabbit with highly purified antigen.
Immunogen Synthetic peptide corresponding to acetylated N-terminal sequence of sheep 14-3-3 eta.Peptide sequence: Ac.GDREQLLQRARC
Shelf Life 18 months from date of despatch.
Gene ID 7533
Other Names 14-3-3 protein eta, Protein AS1, YWHAH, YWHA1
Target/Specificity Rabbit anti-14-3-3η antibody recognizes 14-3-3η in all mammals. It is a member of the 14-3-3 family which consists of 30 kDa proteins that are involved in multiple protein kinase signalling pathways, regulation of cell cycle progression, cytoskeletal structure, transcription, intracellular trafficking and targeting. Protein interactions with 14-3-3 show distinct preference for it’s different isoforms and are regulated by phosphorylation of both 14-3-3 and the bound protein.14-3-3η is expressed principally in the brain although it is expressed at low levels in other tissues. The protein binds alpha-synuclein, forming part of lewy bodies in parkinsons disease and has been linked to early-onset schizophrenia. The eta isoform binds both gremlin 1, which is overexpressed in human cancers, and the DAL-1/Protein 4.1B tumour suppressor. Furthermore the glucocortcoid receptor is positively regulated by 14-3-3- eta. Rabbit anti-14-3-3η antibody may not react with recombinant proteins that are not N-acetylated.
Preservative & Stabilisers 0.09% Sodium Azide
Storage Store at +4℃ or at -20 ℃.
PrecautionsAnti-14-3-3 eta Antibody (Serum) is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
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1. Martin, H. et al. (1993) Antibodies against the major brain isoforms of 14-3-3 protein. An antibody specific for the N-acetylated amino-terminus of a protein.
FEBS Lett. 331 (3): 296-303. 2. Wang, Z. et al. (2011) The prognostic value of 14-3-3 isoforms in vulvar squamous cell carcinoma cases: 14-3-3β and ε are independent prognostic factors for these tumors.
PLoS One. 6: e24843. 3. Taurino, F. et al. (2014) Short-term type-1 diabetes differentially modulates 14-3-3 proteins in rat brain and liver.
Eur J Clin Invest. 44 (4): 350-8. 4. De, S. and Kline, D. (2013) Evidence for the requirement of 14-3-3eta (YWHAH) in meiotic spindle assembly during mouse oocyte maturation.
BMC Dev Biol. 13: 10. 1. Aitken, A. (2006) 14-3-3 proteins: a historic overview.
Semin Cancer Biol. 16 (3): 162-72.

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Cat# ABD10883
(40 western blots)
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