|Application ||WB, IHC-P|
|Calculated MW||28219 Da|
|Purification||Antisera to sheep 14-3-3 eta were raised by repeated immunisations of rabbit with highly purified antigen.|
|Immunogen||Synthetic peptide corresponding to acetylated N-terminal sequence of sheep 14-3-3 eta.Peptide sequence: Ac.GDREQLLQRARC|
|Shelf Life||18 months from date of despatch.|
|Other Names||14-3-3 protein eta, Protein AS1, YWHAH, YWHA1|
|Target/Specificity||Rabbit anti-14-3-3η antibody recognizes 14-3-3η in all mammals. It is a member of the 14-3-3 family which consists of 30 kDa proteins that are involved in multiple protein kinase signalling pathways, regulation of cell cycle progression, cytoskeletal structure, transcription, intracellular trafficking and targeting. Protein interactions with 14-3-3 show distinct preference for it’s different isoforms and are regulated by phosphorylation of both 14-3-3 and the bound protein.14-3-3η is expressed principally in the brain although it is expressed at low levels in other tissues. The protein binds alpha-synuclein, forming part of lewy bodies in parkinsons disease and has been linked to early-onset schizophrenia. The eta isoform binds both gremlin 1, which is overexpressed in human cancers, and the DAL-1/Protein 4.1B tumour suppressor. Furthermore the glucocortcoid receptor is positively regulated by 14-3-3- eta. Rabbit anti-14-3-3η antibody may not react with recombinant proteins that are not N-acetylated.|
|Preservative & Stabilisers||0.09% Sodium Azide|
|Storage||Store at +4℃ or at -20 ℃.|
|Precautions||Anti-14-3-3 eta Antibody (Serum) is for research use only and not for use in diagnostic or therapeutic procedures.|
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Provided below are standard protocols that you may find useful for product applications.
1. Martin, H. et al. (1993) Antibodies against the major brain isoforms of 14-3-3 protein. An antibody specific for the N-acetylated amino-terminus of a protein.FEBS Lett. 331 (3): 296-303. 2. Wang, Z. et al. (2011) The prognostic value of 14-3-3 isoforms in vulvar squamous cell carcinoma cases: 14-3-3β and ε are independent prognostic factors for these tumors.PLoS One. 6: e24843. 3. Taurino, F. et al. (2014) Short-term type-1 diabetes differentially modulates 14-3-3 proteins in rat brain and liver.Eur J Clin Invest. 44 (4): 350-8. 4. De, S. and Kline, D. (2013) Evidence for the requirement of 14-3-3eta (YWHAH) in meiotic spindle assembly during mouse oocyte maturation.BMC Dev Biol. 13: 10. 1. Aitken, A. (2006) 14-3-3 proteins: a historic overview.Semin Cancer Biol. 16 (3): 162-72.
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