|Application ||WB, IHC-P, E|
|Calculated MW||18000 Da|
|Purification||Antisera to sheep 14-3-3 gamma were raised by repeated immunisations of rabbit with highly purified antigen.|
|Immunogen||Synthetic peptide of acetylated N-terminal amino acids 1-10 of sheep 14-3-3 gamma.Peptide sequence: Ac.VDREQLVQKAC|
|Shelf Life||18 months from date of despatch.|
|Other Names||14-3-3 protein gamma, Protein kinase C inhibitor protein 1, KCIP-1, YWHAG|
|Target/Specificity||Rabbit anti-14-3-3γ antibody recognizes the acetylated N-terminal of 14-3-3γ, a member of the 14-3-3 family which consists of 30 kDa proteins involved in multiple protein kinase signalling pathways, regulation of cell cycle progression, cytoskeletal structure, transcription, intracellular trafficking and targeting. Protein interactions with 14-3-3 show distinct preference for it’s different isoforms and are regulated by phosphorylation of both 14-3-3 and the bound protein.14-3-3γ is thought to play an important role in muscle, being induced by growth factors in human vascular smooth muscle cells, and being expressed in skeletal and heart muscles. An example is the gamma isoform binds muscle-specific receptor tyrosine kinase regulating synaptic gene transmission.The gamma isoform binds amyloid beta-protein precursor intracellular domain fragment (AICD) and FE65, facilitating FE65-dependent gene transactivation. The binding of 14-3-3γ to glial fibrillary acidic protein is important in the regulation of glial filaments. 14-3-3 gamma binds and may regulate CDK11 during the cell cycle and apoptosis. The gamma isoform is lowered in foetal Downs syndrome brains.|
|Preservative & Stabilisers||0.09% Sodium Azide|
|Storage||Store at +4℃ or at -20 ℃.|
|Precautions||Anti-14-3-3 gamma Antibody (Serum) is for research use only and not for use in diagnostic or therapeutic procedures.|
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Provided below are standard protocols that you may find useful for product applications.
1. Martin, H. et al. (1993) Antibodies against the major brain isoforms of 14-3-3 protein. An antibody specific for the N-acetylated amino-terminus of a protein.FEBS Lett. 331 (3): 296-303. 2. Wang, Z. et al. (2011) The prognostic value of 14-3-3 isoforms in vulvar squamous cell carcinoma cases: 14-3-3β and ε are independent prognostic factors for these tumors.PLoS One. 6: e24843. 3. Taurino, F. et al. (2014) Short-term type-1 diabetes differentially modulates 14-3-3 proteins in rat brain and liver.Eur J Clin Invest. 44 (4): 350-8. 4. De, S. et al. (2012) Expression of 14-3-3 protein isoforms in mouse oocytes, eggs and ovarian follicular developmentBMC Res Notes. 5: 57.1. Aitken, A. (2006) 14-3-3 proteins: a historic overview.Semin Cancer Biol. 16 (3): 162-72.
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