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Anti-14-3-3 gamma Antibody (Serum)

Rabbit Anti-Sheep Polyclonal Antibody

     
  • IF - Anti-14-3-3 gamma Antibody (Serum) ABD10884
    Published customer image:Representative immunofluorescence images of 14-3-3 isoforms in oocytes and eggs isolated from adult mice. (A, B) 14-3-3β. (C, D) 14-3-3γ. (E, F) 14-3-3ε. (G, H) 14-3-3ζ. (I, J) 14-3-3 τ. (K, L) 14-3-3σ. (M, N) 14-3-3η. Confocal sections with regions of red fluorescence indicating the corresponding isoforms studied (see Methods). The inset in N shows the same egg labeled blue with Hoechst DNA stain (non-confocal image) and confirms that the darker areas in this region of the larger image are condensed metaphase II chromosomes. Control cells were included for each isoform experiment and were imaged using the same confocal settings. Representative control oocytes and eggs are shown in bright-field (O) and fluorescence (P). 14-3-3η accumulates, in part, in the meiotic spindle in eggs as shown by simultaneous labeling with 14-3-3η (Q) and tubulin (R) antibodies. These sequential scans are merged (14-3-3η + tubulin) in (S). The scale bars represent 10 µm for all images.From: De S, Marcinkiewicz JL, Vijayaraghavan S, Kline D. Expression of 14-3-3 protein isoforms in mouse oocytes, eggs and ovarian follicular development. BMC Res Notes. 2012 Jan 23;5:57.
  • IHC - Anti-14-3-3 gamma Antibody (Serum) ABD10884
    Published customer image:The expression of 14-3-3 proteins in the vulvar squamous epithelium. Representative samples stained using immunohistochemistry are shown. The expression of the 14-3-3 proteins (β, γ, ζ, ε, η and τ) in normal vulvar squamous epithelium (A–F). The increased cytoplasmic expression of 14-3-3β, γ, ζ, ε and η (G–K) and the reduced nuclear expression of 14-3-3τ (L) in vulvar carcinomas.From: Wang Z, Nesland JM, Suo Z, Trope CG, Holm R The Prognostic Value of 14-3-3 Isoforms in Vulvar Squamous Cell Carcinoma Cases: 14-3-3β and ε Are Independent Prognostic Factors for These Tumors. PLoS ONE 6(9): e24843.
  • IHC - Anti-14-3-3 gamma Antibody (Serum) ABD10884
    Published customer image:Representative immunohistochemistry images of 14-3-3 γ in the different stages of follicular development in ovarian sections. (A) Primordial follicle. (B) Primary follicle. (C) Secondary follicle. (D) Early antral follicle. (E) Graafian (advanced antral) follicle. (F) Corpus luteum. White arrows indicate the primordial or primary follicles in (A and B). Note the weaker staining in mural granulosa cells in secondary follicles (C, green arrows) and the more intense staining in cells lining the antral cavity (D and E, red arrows). The scale bars represent 10 µm (A-C) or 100 µm (D-F).From: De S, Marcinkiewicz JL, Vijayaraghavan S, Kline D. Expression of 14-3-3 protein isoforms in mouse oocytes, eggs and ovarian follicular development. BMC Res Notes. 2012 Jan 23;5:57.
  • WB - Anti-14-3-3 gamma Antibody (Serum) ABD10884
    Western blots of 14-3-3 isoforms in HEK293 cell extracts using AbD Serotec antibodies.Lane 1 = anti-gamma 14-3-3 Lane 2 = anti-zeta 14-3-3 Lane 3 = anti-C-epsilon-14-3-3 Lane 4 = anti-N-epsilon-14-3-3 Lane 5 = anti-eta 14-3-3. The 14-3-3 isoforms run at 30 kDa except epsilon 14-3-3 which migrates at 33 kDa
  • IHC - Anti-14-3-3 gamma Antibody (Serum) ABD10884
    Published customer image:Representative immunohistochemistry images of 14-3-3 protein isoforms in atretic follicles of adult mouse ovaries. (A) 14-3-3 β. (B) 14-3-3 γ. (C) 14-3-3 ε. (D) 14-3-3 ζ. (E) 14-3-3η. (F) 14-3-3 τ. (G) 14-3-3 σ. All scale bars indicate 100 µm.From: De S, Marcinkiewicz JL, Vijayaraghavan S, Kline D. Expression of 14-3-3 protein isoforms in mouse oocytes, eggs and ovarian follicular development. BMC Res Notes. 2012 Jan 23;5:57.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, E
Primary Accession P68253
Reactivity Sheep
Host Rabbit
Clonality Polyclonal
Isotype Polyclonal IgG
Calculated MW 18000 Da
Additional Information
Other Species M,H,B,C,Rat
Purification Antisera to sheep 14-3-3 gamma were raised by repeated immunisations of rabbit with highly purified antigen.
Immunogen Synthetic peptide of acetylated N-terminal amino acids 1-10 of sheep 14-3-3 gamma.Peptide sequence: Ac.VDREQLVQKAC
Shelf Life 18 months from date of despatch.
Other Names 14-3-3 protein gamma, Protein kinase C inhibitor protein 1, KCIP-1, YWHAG
Target/Specificity Rabbit anti-14-3-3γ antibody recognizes the acetylated N-terminal of 14-3-3γ, a member of the 14-3-3 family which consists of 30 kDa proteins involved in multiple protein kinase signalling pathways, regulation of cell cycle progression, cytoskeletal structure, transcription, intracellular trafficking and targeting. Protein interactions with 14-3-3 show distinct preference for it’s different isoforms and are regulated by phosphorylation of both 14-3-3 and the bound protein.14-3-3γ is thought to play an important role in muscle, being induced by growth factors in human vascular smooth muscle cells, and being expressed in skeletal and heart muscles. An example is the gamma isoform binds muscle-specific receptor tyrosine kinase regulating synaptic gene transmission.The gamma isoform binds amyloid beta-protein precursor intracellular domain fragment (AICD) and FE65, facilitating FE65-dependent gene transactivation. The binding of 14-3-3γ to glial fibrillary acidic protein is important in the regulation of glial filaments. 14-3-3 gamma binds and may regulate CDK11 during the cell cycle and apoptosis. The gamma isoform is lowered in foetal Downs syndrome brains.
Preservative & Stabilisers 0.09% Sodium Azide
Storage Store at +4℃ or at -20 ℃.
PrecautionsAnti-14-3-3 gamma Antibody (Serum) is for research use only and not for use in diagnostic or therapeutic procedures.
Citations (0)

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References

1. Martin, H. et al. (1993) Antibodies against the major brain isoforms of 14-3-3 protein. An antibody specific for the N-acetylated amino-terminus of a protein.
FEBS Lett. 331 (3): 296-303. 2. Wang, Z. et al. (2011) The prognostic value of 14-3-3 isoforms in vulvar squamous cell carcinoma cases: 14-3-3β and ε are independent prognostic factors for these tumors.
PLoS One. 6: e24843. 3. Taurino, F. et al. (2014) Short-term type-1 diabetes differentially modulates 14-3-3 proteins in rat brain and liver.
Eur J Clin Invest. 44 (4): 350-8. 4. De, S. et al. (2012) Expression of 14-3-3 protein isoforms in mouse oocytes, eggs and ovarian follicular development
BMC Res Notes. 5: 57.1. Aitken, A. (2006) 14-3-3 proteins: a historic overview.
Semin Cancer Biol. 16 (3): 162-72.

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