|Other Accession||P68253, P29361, P68251, O77642|
|Calculated MW||29174 Da|
|Purification||Antisera to Phosphorylated 14-3-3 were raised by repeated immunisations of sheep with highly purified antigen. Purified IgG prepared by affinity chromatography.|
|Immunogen||Synthetic peptide corresponding to 14-3-3 beta and zeta phosphorylated at serine 185.Peptide sequence: EILNSPEKAC|
|Shelf Life||18 months from the date of despatch.|
|Other Names||14-3-3 protein epsilon, 14-3-3E, Protein kinase C inhibitor protein 1, KCIP-1, YWHAE|
|Target/Specificity||Sheep anti-phosphorylated 14-3-3 antibody recognises the 14-3-3 isoforms beta and zeta which are phosphorylated at serine 185. The members of the 14-3-3 family of proteins are highly conserved and are involved in multiple signalling pathways. The phosphorylated forms of beta and zeta 14-3-3 are found endogenously in brain tissue.|
|Preservative & Stabilisers||0.09% Sodium Azide|
|Storage||Store at +4℃ or at -20 ℃.|
|Precautions||Anti-14-3-3 (pSer185) Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
email@example.com, and receive a free "I Love Antibodies" mug.
Provided below are standard protocols that you may find useful for product applications.
1. Aitken, A. et al. (1995) 14-3-3 alpha and delta are the phosphorylated forms of Raf-activating 14-3-3 beta and zeta. In vivo stoichiometric phosphorylation in brain at Ser-Pro-Glu-Lys Motif. J. Biol. Chem. 270: 5706 – 5709.
If you have used an Abgent product and would like to share how it has performed, please click on the "Submit Review" button and provide the requested information. Our staff will examine and post your review and contact you if needed.
If you have any additional inquiries please email technical services at firstname.lastname@example.org.