|Other Accession||P68253, P29361, P68251, O77642|
|Calculated MW||29174 Da|
|Purification||Antisera to Phosphorylated 14-3-3 were raised by repeated immunisations of sheep with highly purified antigen. Purified IgG prepared by affinity chromatography.|
|Immunogen||Synthetic peptide corresponding to 14-3-3 beta and zeta phosphorylated at serine 185.Peptide sequence: EILNSPEKAC|
|Shelf Life||18 months from the date of despatch.|
|Other Names||14-3-3 protein epsilon, 14-3-3E, Protein kinase C inhibitor protein 1, KCIP-1, YWHAE|
|Target/Specificity||Sheep anti-phosphorylated 14-3-3 antibody recognises the 14-3-3 isoforms beta and zeta which are phosphorylated at serine 185. The members of the 14-3-3 family of proteins are highly conserved and are involved in multiple signalling pathways. The phosphorylated forms of beta and zeta 14-3-3 are found endogenously in brain tissue.|
|Preservative & Stabilisers||0.09% Sodium Azide|
|Storage||Store at +4℃ or at -20 ℃.|
|Precautions||Anti-14-3-3 (pSer185) Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
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Provided below are standard protocols that you may find useful for product applications.
1. Aitken, A. et al. (1995) 14-3-3 alpha and delta are the phosphorylated forms of Raf-activating 14-3-3 beta and zeta. In vivo stoichiometric phosphorylation in brain at Ser-Pro-Glu-Lys Motif. J. Biol. Chem. 270: 5706 – 5709.
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