|Application ||WB, IHC-P, E|
|Purification||Antisera to sheep 14-3-3 tau/theta were raised by repeated immunisations of rabbit with highly purified antigen.|
|Immunogen||Synthetic peptide corresponding to acetylated N-terminal sequence of sheep 14-3-3 tau/theta.Peptide sequence: Ac.MEKTELIQKAC|
|Shelf Life||18 months from date of despatch.|
|Target/Specificity||Rabbit anti-14-3-3 τ/θ antibody recognizes 14-3-3 tau/theta in all mammals. It is a member of the 14-3-3 family which consists of 30 kDa proteins involved in multiple cell signalling pathways, regulation of cell cycle progression, cytoskeletal structure, transcription, intracellular trafficking and targeting. Protein interactions with 14-3-3 show distinct preferences for it’s different isoforms and are regulated by phosphorylation of both 14-3-3 and the bound protein.14-3-3 tau/theta is primarily expressed in T-cells although it occurs at lower levels in the brain, heart, pancreas placenta and kidneys. It plays a role in cell cycle progression via interaction with p27(Kip1). Along with COPI, the theta isoform binds the GB1 RSR sequence involved in protein trafficking. The isoform forms a complex with Hsp60 and cellular prion protein which may be involved in prion diseases. Elevated levels of 14-3-3 tau/theta are observed in amyotrophic lateral sclerosis. 14-3-3 tau/theta also binds BCR/Abl, bacterial effector protein Tir, yes-associated protein and the FSH receptor.Rabbit anti-14-3-3 τ/θ antibody may not react with recombinant proteins that are not N-acetylated.|
|Preservative & Stabilisers||0.09% Sodium Azide|
|Storage||Store at +4℃ or at -20 ℃.|
|Precautions||Anti-14-3-3 tau/theta Antibody (Serum) is for research use only and not for use in diagnostic or therapeutic procedures.|
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Provided below are standard protocols that you may find useful for product applications.
1. Martin, H. et al. (1993) Antibodies against the major brain isoforms of 14-3-3 protein. An antibody specific for the N-acetylated amino-terminus of a protein.FEBS Lett. 331 (3): 296-303. 2. Wang, Z. et al. (2011) The prognostic value of 14-3-3 isoforms in vulvar squamous cell carcinoma cases: 14-3-3β and ε are independent prognostic factors for these tumors.PLoS One. 6: e24843. 3. Taurino, F. et al. (2014) Short-term type-1 diabetes differentially modulates 14-3-3 proteins in rat brain and liver.Eur J Clin Invest. 44 (4): 350-8. 4. De, S. et al. (2012) Expression of 14-3-3 protein isoforms in mouse oocytes, eggs and ovarian follicular developmentBMC Res Notes. 5: 57.1. Aitken, A. (2006) 14-3-3 proteins: a historic overview.Semin Cancer Biol. 16 (3): 162-72.
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