|Application ||WB, IHC-P, E|
|Calculated MW||27856 Da|
|Purification||Antisera to anti 14-3-3 zeta/delta were raised by repeated immunisations of rabbits with highly purified antigen.|
|Immunogen||Synthetic peptide corresponding to acetylated N-terminal sequence of sheep 14-3-3 zeta/delta.Peptide sequence: Ac.MDKNELVQKAC|
|Shelf Life||18 months from date of despatch.|
|Other Names||14-3-3 protein zeta/delta, Protein kinase C inhibitor protein 1, KCIP-1, YWHAZ|
|Target/Specificity||Rabbit anti-14-3-3ζ antibody recognizes the acetylated N-terminal of 14-3-3 zeta and the phosphorylated zeta isoform (also known as 14-3-3 delta) in all mammals. These are members of the 14-3-3 family which consists of 30 kDa proteins involved in multiple protein kinase signaling pathways, regulation of cell cycle progression, cytoskeletal structure, transcription, intracellular trafficking and targeting. Protein interactions with 14-3-3 show distinct preference for its different isotypes and are regulated by phosphorylation of both 14-3-3 and the bound protein. 14-3-3ζ is a susceptibility gene for paranoid schizophrenia and is overexpressed in the temporal cortex of cognitively impared Alzheimers patients. The protein binds yeast Sps1/Ste20-related kinase 1 (YSK1) and localizes to the golgi, possibly linking YSK1 signaling, protein transport, cell adhesion and migration. It co-localizes with epidermal growth factor receptor to the plasma membrane following epidermal growth factor signaling. The zeta isoform is involved in MAPKAPK2-mediated phosphorylation which may have a role in p38 MAPK-dependent inflammation. When bound to ADAM 22, 14-3-3 zeta is involved in cell adhesion. 14-3-3ζ also interacts with cofilin, LIM-domain-containing protein kinase 1, protein kinase B/Akt and GPI alpha. 14-3-3ζ can be phosphorylated at serine 184 to produce 14-3-3 delta.Rabbit anti-14-3-3ζ antibody may not react with recombinant proteins that are not N-acetylated.|
|Preservative & Stabilisers||0.09% Sodium Azide|
|Storage||Store at +4℃ or at -20 ℃.|
|Precautions||Anti-14-3-3 zeta/delta Antibody (Serum) is for research use only and not for use in diagnostic or therapeutic procedures.|
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Provided below are standard protocols that you may find useful for product applications.
1. Martin, H. et al. (1993) Antibodies against the major brain isoforms of 14-3-3 protein. An antibody specific for the N-acetylated amino-terminus of a protein.FEBS Lett. 331 (3): 296-303. 2. Wang, Z. et al. (2011) The prognostic value of 14-3-3 isoforms in vulvar squamous cell carcinoma cases: 14-3-3β and ε are independent prognostic factors for these tumors.PLoS One. 6: e24843. 3. Piesiewicz, A. et al. (2012) Pineal arylalkylamine N-acetyltransferase (Aanat) gene expression as a target of inflammatory mediators in the chicken.Gen Comp Endocrinol. 179 (2): 143-51. 4. Taurino, F. et al. (2014) Short-term type-1 diabetes differentially modulates 14-3-3 proteins in rat brain and liver.Eur J Clin Invest. 44 (4): 350-8. 5. De, S. et al. (2012) Expression of 14-3-3 protein isoforms in mouse oocytes, eggs and ovarian follicular developmentBMC Res Notes. 5: 57.1. Aitken, A. (2006) 14-3-3 proteins: a historic overview.Semin Cancer Biol. 16 (3): 162-72.
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