|Application ||WB, IHC-P, E|
|Calculated MW||27856 Da|
|Purification||Antisera to anti 14-3-3 zeta/delta were raised by repeated immunisations of rabbits with highly purified antigen.|
|Immunogen||Synthetic peptide corresponding to acetylated N-terminal sequence of sheep 14-3-3 zeta/delta.Peptide sequence: Ac.MDKNELVQKAC|
|Shelf Life||18 months from date of despatch.|
|Other Names||14-3-3 protein zeta/delta, Protein kinase C inhibitor protein 1, KCIP-1, YWHAZ|
|Target/Specificity||Rabbit anti-14-3-3ζ antibody recognizes the acetylated N-terminal of 14-3-3 zeta and the phosphorylated zeta isoform (also known as 14-3-3 delta) in all mammals. These are members of the 14-3-3 family which consists of 30 kDa proteins involved in multiple protein kinase signaling pathways, regulation of cell cycle progression, cytoskeletal structure, transcription, intracellular trafficking and targeting. Protein interactions with 14-3-3 show distinct preference for its different isotypes and are regulated by phosphorylation of both 14-3-3 and the bound protein. 14-3-3ζ is a susceptibility gene for paranoid schizophrenia and is overexpressed in the temporal cortex of cognitively impared Alzheimers patients. The protein binds yeast Sps1/Ste20-related kinase 1 (YSK1) and localizes to the golgi, possibly linking YSK1 signaling, protein transport, cell adhesion and migration. It co-localizes with epidermal growth factor receptor to the plasma membrane following epidermal growth factor signaling. The zeta isoform is involved in MAPKAPK2-mediated phosphorylation which may have a role in p38 MAPK-dependent inflammation. When bound to ADAM 22, 14-3-3 zeta is involved in cell adhesion. 14-3-3ζ also interacts with cofilin, LIM-domain-containing protein kinase 1, protein kinase B/Akt and GPI alpha. 14-3-3ζ can be phosphorylated at serine 184 to produce 14-3-3 delta.Rabbit anti-14-3-3ζ antibody may not react with recombinant proteins that are not N-acetylated.|
|Preservative & Stabilisers||0.09% Sodium Azide|
|Storage||Store at +4℃ or at -20 ℃.|
|Precautions||Anti-14-3-3 zeta/delta Antibody (Serum) is for research use only and not for use in diagnostic or therapeutic procedures.|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
1. Martin, H. et al. (1993) Antibodies against the major brain isoforms of 14-3-3 protein. An antibody specific for the N-acetylated amino-terminus of a protein.FEBS Lett. 331 (3): 296-303. 2. Wang, Z. et al. (2011) The prognostic value of 14-3-3 isoforms in vulvar squamous cell carcinoma cases: 14-3-3β and ε are independent prognostic factors for these tumors.PLoS One. 6: e24843. 3. Piesiewicz, A. et al. (2012) Pineal arylalkylamine N-acetyltransferase (Aanat) gene expression as a target of inflammatory mediators in the chicken.Gen Comp Endocrinol. 179 (2): 143-51. 4. Taurino, F. et al. (2014) Short-term type-1 diabetes differentially modulates 14-3-3 proteins in rat brain and liver.Eur J Clin Invest. 44 (4): 350-8. 5. De, S. et al. (2012) Expression of 14-3-3 protein isoforms in mouse oocytes, eggs and ovarian follicular developmentBMC Res Notes. 5: 57.1. Aitken, A. (2006) 14-3-3 proteins: a historic overview.Semin Cancer Biol. 16 (3): 162-72.
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