|Application ||WB, IHC-P, E|
|Calculated MW||40076 Da|
|Purification||Antisera to CD14 were raised by repeated immunisations of goat with highly purified antigen. Purified IgG prepared by affinity chromatography using the immunizing peptide, reactivity with the carrier protein was removed during purification.|
|Immunogen||Peptide with sequence C-KRVDADADPRQYAD corresponding to amino acid residues 71-84 of the human CD14 precursor conjugated to a carrier protein.|
|Shelf Life||18 months from date of despatch.|
|Other Names||Monocyte differentiation antigen CD14, Myeloid cell-specific leucine-rich glycoprotein, CD14, Monocyte differentiation antigen CD14, urinary form, Monocyte differentiation antigen CD14, membrane-bound form, CD14|
|Target/Specificity||Goat anti-Human CD14 antibody recognizes human CD14, a 55 kDa receptor for endotoxin and other bacterial cell wall components.Goat anti-Human CD14 antibody detects an epitope within the internal region of CD14. CD14 acts as a multiligand pattern recognition receptor, inducing specific coassembly of additional receptors, including MD-2 and TLR4 as part of the lipopolysaccharide (LPS) receptor.The LPS receptor is expressed strongly on the surface of monocytes and weakly on the surface of granulocytes, and is also expressed by most tissue macrophages. Activation of the LPS receptor leads to NF-kappa-B activation, cytokine secretion and the inflammatory response. CD14 also up-regulates cell surface molecules, including adhesion molecules, and has been shown to bind apoptotic cells|
|Preservative & Stabilisers||0.02% Sodium Azide (NaN3); 0.5% Bovine Serum Albumin;|
|Storage||Store at +4℃ or at -20 ℃.|
|Precautions||Anti-Human CD14 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
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Provided below are standard protocols that you may find useful for product applications.
1. Triantafilou, M. and Triantafilou, K. (2002) Lipopolysaccharide recognition: CD14, TLRs and the LPS-activation cluster. Trends Immunol. 23: 301-304. 2. Miyake, K. (2003) Innate recognition of lipopolysaccharide by CD14 and toll-like receptor 4-MD-2: unique roles for MD-2.Int. Immunopharmacol. 3: 119-128.
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