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Anti-Human GAPDH (C-Terminal) Antibody

Goat Anti-Human Polyclonal Antibody

Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P04406
Reactivity Human
Host Goat
Clonality Polyclonal
Isotype Polyclonal IgG
Calculated MW 36053 Da
Additional Information
Other Species Dog,M,Pig,Rat
Purification Antisera to human GAPDH (CT) were raised by repeated immunisations of goat with highly purified antigen. Purified IgG prepared by affinity chromatography.
Immunogen Peptide sequence C-HQVVSSDFNSDT corresponding to the C-terminal region of GAPDH (NP_002037.2).
Shelf Life 18 months from date of despatch.
Gene ID 2597
Other Names Glyceraldehyde-3-phosphate dehydrogenase, GAPDH,, Peptidyl-cysteine S-nitrosylase GAPDH, 2.6.99.-, GAPDH, GAPD
Target/Specificity Goat anti-Human GAPDH antibody recognizes an epitope within the C-terminal (CT) region of glyceraldehyde-3-phosphate dehydrogenase (GAPDH), a ~36 kDa multifunctional protein whose main function is to catalyse the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate, in conjunction with inorganic phosphate and nicotinamide adenine dinucleotide (NAD). This reaction is an important energy yielding step in carbohydrate metabolism. GAPDH translocates to the nucleus under a variety of stressors, most of which are associated with oxidative stress, whereby it mediates cell death(Dastooret al. 2001). GAPDH binds to several proteins that are responsible for neurodegenerative diseases, such as amyloid precursor protein and Huntingtin (Burkeet al.1996).
Preservative & Stabilisers 0.02% Sodium Azide (NaN3); 0.5% Bovine Serum Albumin;
Storage Store at +4℃ or at -20 ℃.
PrecautionsAnti-Human GAPDH (C-Terminal) Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
Citations (0)

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1. Hara, M.R. et al. (2006) GAPDH as a sensor of NO stress.
Biochim. Biophys. Acta. 1762: 502-509. 2. Mazzola, J.L. and Sirover, M.A (2001) Reduction of glyceraldehyde-3-phosphate dehydrogenase activity in Alzheimer’s disease and in Huntington’s disease fibroblasts.
J. Neurochem. 76: 442-449. 3. Wang H et al. (2013) Hypoxic preconditioning alleviates ethanol neurotoxicity: the involvement of autophagy.
Neurotox Res. 24 (4): 472-7.

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Cat# ABD10894
(40 western blots)
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