|Application ||WB, IHC-F, IF, E|
|Calculated MW||56812 Da|
|Purification||Antisera to alkaline phosphatase were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG prepared by ion exchange chromatography.|
|Immunogen||Full length E.coli alkaline phosphatase.|
|Shelf Life||18 months from date of despatch.|
|Other Names||Intestinal-type alkaline phosphatase, IAP, Intestinal alkaline phosphatase, 22.214.171.124, ALPI|
|Target/Specificity||Rabbit anti-Bacterial Alkaline Phosphatase antibody detects bacterial alkaline phosphatase (BAP), a periplasmic enzyme which catalyzes the release of 5'- and 3'-phosphate groups from DNA, RNA and nucleotides. BAP can also dephosphorylate a number of proteins and alkaloids, and has an optimum pH of 8.0. BAP is encoded by the phoA gene, and forms a homodimer of two identical ~47 kDa subunits.It is thought that bacteria use BAP to create free phosphate groups for uptake and use. Other functions include dephosphorylating extracellular organic compounds for import into the cell.Rabbit anti-Bacterial Alkaline Phosphatase antibody does not react with mammalian forms of alkaline phosphatase.|
|Preservative & Stabilisers||0.01% Gentamicin Sulphate; 1% Bovine Serum Albumin;|
|Storage||Store at +4℃ or at -20 ℃.|
|Precautions||Anti-Bacterial Alkaline Phosphatase Antibody (HRP) is for research use only and not for use in diagnostic or therapeutic procedures.|
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Provided below are standard protocols that you may find useful for product applications.
1. Bradshaw, R.A. et al. (1981) Amino Acid Sequence of Escherichia coli Alkaline Phosphatase. PNAS. 78: 3473 - 3477. 2. Wyckoff HW. (1987) Structure of Escherichia coli alkaline phosphatase determined by X- ray diffraction. Phosphate Metabolism and Cellular Regulation in Microorganisms. Edited by: Torriani – Gorini, A., Rothman, FG., Silver, S., Wright, A. and Yagil, E. America Society for Microbiology, Washington, DC. pp. 118–126. 3. Akiyama, I. and Ito, K. (1993) Folding and assembly of bacterial alkaline phosphatase in vitro and in vivo. J. Biol. Chem. 268: 8146 - 8150.
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