|Application ||WB, IHC-F, IF|
|Purification||Antisera to bovine smooth muscle myosin II were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG prepared by ion exchange chromatography.|
|Immunogen||Bovine tracheal smooth muscle myosin.|
|Shelf Life||12 months from date of reconstitution.|
|Target/Specificity||Rabbit anti-Bovine Smooth Muscle Myosin II antibody recognizes the heavy chain of myosin II found in vertebrate smooth muscle, a non-striated muscle present in the walls of hollow organs, the vasculature, the respiratory and gastrointestinal tracts, uterus, bladder and eye.The structure of myosin II is fundamentally the same in all muscle types and consists of two heavy chains, each bound by 2 distinct, essential and regulatory light chains. The globular motor domain located at the N-terminal domain of the heavy chains, catalyzes ATP hydrolysis and interacts with actin, whilst the heptad repeat sequences of the heavy chain tail domains promote dimerization, forming an alpha-helical coiled coil at the C-terminal domain. Rabbit anti-Bovine Smooth Muscle Myosin II antibody does not cross-react with myosin of skeletal or non-muscle origin and can be used to specifically stain cultured smooth muscle cells and identify vascular and other smooth muscle types in tissue sections.|
|Preservative & Stabilisers||0.006% Sodium Azide (NaN3)|
|Storage||Store at -20℃ only.|
|Precautions||Anti-Bovine Smooth Muscle Myosin II Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
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Provided below are standard protocols that you may find useful for product applications.
1. Xiao, Q. et al. (2011) Chromobox protein homolog 3 is essential for stem cell differentiation to smooth muscles in vitro and in embryonic arteriogenesis.Arterioscler Thromb Vasc Biol. 31: 1842-52. 2. Zhang, L. et al. (2010) Sp1-dependent activation of HDAC7 is required for platelet-derived growth factor-BB-induced smooth muscle cell differentiation from stem cells.J Biol Chem. 285 (49): 38463-72. 3. Margariti, A. et al. (2009) Splicing of HDAC7 modulates the SRF-myocardin complex during stem-cell differentiation towards smooth muscle cells.J Cell Sci. 122: 460-70. 4. Xiao, Q. et al. (2009) Embryonic stem cell differentiation into smooth muscle cells is mediated by Nox4-produced H2O2.Am J Physiol Cell Physiol. 296: C711-23. 5. Wang, G. et al. (2012) Functional impact of heterogeneous nuclear ribonucleoprotein A2/B1 in smooth muscle differentiation from stem cells and embryonic arteriogenesis.J Biol Chem. 287 (4): 2896-906. 6. Zheng, X. et al. (2013) Angiotensin II promotes differentiation of mouse embryonic stem cells to smooth muscle cells through PI3-kinase signaling pathway and NF-κB.Differentiation. 85: 41-54.1. Rosenfeld, S.S. et al. (1998) Structural and kinetic studies of phosphorylation-dependent regulation in smooth muscle myosin.J Biol Chem. 273 (44): 28682-90.
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