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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   Anti-Darpp-32 (N-Terminal) Antibody    

Anti-Darpp-32 (N-Terminal) Antibody

Rabbit Anti-Rat Polyclonal Antibody

  • WB - Anti-Darpp-32 (N-Terminal) Antibody  ABD10927
    Western blot of the rat hippocampal lysate showing specific immunolabeling of the ~32 kDa DARPP protein
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q6J4I0
Reactivity Rat
Host Rabbit
Clonality Polyclonal
Isotype Polyclonal IgG
Calculated MW 22913 Da
Additional Information
Purification Antisera to rat DARPP-32 (NT) were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
Immunogen Peptide corresponding to the N-terminal region of rat DARPP-32, conjugated to keyhole limpet haemocyanin (KLH).
Shelf Life 12 months from date of reconstitution.
Gene ID 360616
Other Names Protein phosphatase 1 regulatory subunit 1B, DARPP-32, Dopamine- and cAMP-regulated neuronal phosphoprotein, Ppp1r1b
Target/Specificity Rabbit anti-Rat DARPP-32 antibody recognizes rat Protein phosphatase 1 regulatory subunit 1B, also known as DARPP-32 or Dopamine- and cAMP-regulated neuronal phosphoprotein. DARPP-32 is a 205 amino acid ~32 kDa member of the protein phosphatase inhibitor 1 family. Originally discovered as a substrate for cAMP-dependent protein kinase which modulates cell signalling in neurons. DARPP-32 is converted into an inhibitor of protein phosphatase-1 when phosphorylated by protein kinase A at threonine 34, as a result of dopaminergic receptor stimulation (Bibbet al.1999). However this phosphorylation is reversed on stimulation of N-methyl-D-aspartate (NMDA) receptors by glutamate (Halpainet al.1990). Conversely when phosphorylated at threonine-75 by CDK5, DARPP-32 becomes an inhibitor of protein kinase A (Bibbet al.1999).DARPP-32 is highly expressed in striatonigral and striatopallidal projection neurons but is not observed in cholinergic and neuropeptide Y-containing striatal interneurons (Anderson and Reiner 1991). A truncated form of DARPP-32, t-DARPP is over-expressed in gastric cancer cells (El-Rifaiet al.2002) and, further t-DARPP has subsequently been identified as overexpressed in Her-2 positive breast cancer cells and implicated in resistance of these cells to Herceptin treament (Guet al.2009).DARPP-32 appears to have evolved early in vertebrate evolution and may have first appeared in gnathostomes. The immunogen sequence for Rabbit anti-rat DARPP-32 antibody is identical in canine, chicken, human, mouse and Xenopus, therefore the antibody therefore is expected to recognize DARP-32 in all these species. Rabbit anti-rat DARPP-32 antibody is not expected to recognize t-DARPP as this lacks the amino sequence used for immunization. For the identification of specific phospho DARPP-32 forms the use af antibodies against the threonine 34 and 75 phospho-epitopes is recommended.
Preservative & Stabilisers 0.09% Sodium Azide; 0.01% Bovine Serum Albumin; 50% Glycerol
Storage Store at +4℃ or at -20 ℃.
PrecautionsAnti-Darpp-32 (N-Terminal) Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
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1. Bibb, J. A. et al. (1999) Phosphorylation of DARPP-32 by Cdk5 modulates dopamine signalling in neurons.
Nature 402:669-671. 2. Fienberg, A. et al. (1998) DARPP-32: regulator of the efficacy of dopaminergic neurotransmission.
Science 281:838-842.

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Cat# ABD10927
(40 western blots)
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