|Calculated MW||22913 Da|
|Purification||Antisera to rat DARPP-32 (NT) were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.|
|Immunogen||Peptide corresponding to the N-terminal region of rat DARPP-32, conjugated to keyhole limpet haemocyanin (KLH).|
|Shelf Life||12 months from date of reconstitution.|
|Other Names||Protein phosphatase 1 regulatory subunit 1B, DARPP-32, Dopamine- and cAMP-regulated neuronal phosphoprotein, Ppp1r1b|
|Target/Specificity||Rabbit anti-Rat DARPP-32 antibody recognizes rat Protein phosphatase 1 regulatory subunit 1B, also known as DARPP-32 or Dopamine- and cAMP-regulated neuronal phosphoprotein. DARPP-32 is a 205 amino acid ~32 kDa member of the protein phosphatase inhibitor 1 family. Originally discovered as a substrate for cAMP-dependent protein kinase which modulates cell signalling in neurons. DARPP-32 is converted into an inhibitor of protein phosphatase-1 when phosphorylated by protein kinase A at threonine 34, as a result of dopaminergic receptor stimulation (Bibbet al.1999). However this phosphorylation is reversed on stimulation of N-methyl-D-aspartate (NMDA) receptors by glutamate (Halpainet al.1990). Conversely when phosphorylated at threonine-75 by CDK5, DARPP-32 becomes an inhibitor of protein kinase A (Bibbet al.1999).DARPP-32 is highly expressed in striatonigral and striatopallidal projection neurons but is not observed in cholinergic and neuropeptide Y-containing striatal interneurons (Anderson and Reiner 1991). A truncated form of DARPP-32, t-DARPP is over-expressed in gastric cancer cells (El-Rifaiet al.2002) and, further t-DARPP has subsequently been identified as overexpressed in Her-2 positive breast cancer cells and implicated in resistance of these cells to Herceptin treament (Guet al.2009).DARPP-32 appears to have evolved early in vertebrate evolution and may have first appeared in gnathostomes. The immunogen sequence for Rabbit anti-rat DARPP-32 antibody is identical in canine, chicken, human, mouse and Xenopus, therefore the antibody therefore is expected to recognize DARP-32 in all these species. Rabbit anti-rat DARPP-32 antibody is not expected to recognize t-DARPP as this lacks the amino sequence used for immunization. For the identification of specific phospho DARPP-32 forms the use af antibodies against the threonine 34 and 75 phospho-epitopes is recommended.|
|Preservative & Stabilisers||0.09% Sodium Azide; 0.01% Bovine Serum Albumin; 50% Glycerol|
|Storage||Store at +4℃ or at -20 ℃.|
|Precautions||Anti-Darpp-32 (N-Terminal) Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
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