|Application ||WB, IHC-F|
|Calculated MW||18016 Da|
|Purification||Antisera to Bik were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG prepared by affinity chromatography.|
|Immunogen||15 amino acid peptide from near the amino terminus of human Bik.|
|Shelf Life||18 months from date of despatch.|
|Other Names||Bcl-2-interacting killer, Apoptosis inducer NBK, BIP1, BP4, BIK, NBK|
|Target/Specificity||ABD10936 detects an epitope within the N-terminal (NT) region of Bcl-2-interacting killer (Bik) also known as natural born killer (Nbk). Bik is a Bcl-2 homology domain 3 (BH3)-only protein of 18kD which requires the BAX gene for activation. Bik forms heterodimers with various antiapoptotic proteins such as Bcl-2 and Bcl-XL, inhibiting their antiapoptotic function. This can result in the release of Ca2+ and cytochrome C into the cytosol, leading to apoptosis. Loss of Bik is a common feature of clear-cell renal cell carcinoma (RCC), and is thought to act as a renal tissue-specific tumour suppressor gene.|
|Preservative & Stabilisers||0.02% Sodium Azide|
|Storage||Store at +4℃ or at -20 ℃.|
|Precautions||Anti-BIK (N-Terminal) Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
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Provided below are standard protocols that you may find useful for product applications.
1. Mathai, J.P. et al. (2005) BH3-only BIK regulates BAX,BAK-dependent release of Ca2+ from endoplasmic reticulum stores and mitochondrial apoptosis during stress-induced cell death. J. Biol. Chem. 280: 23829-23836.2. Real, P.J. et al. (2006) Transcriptional activation of the proapoptotic bik gene by E2F proteins in cancer cells.FEBS Lett. 580: 5905-5909.3. Sturm, I. et al. (2006) Loss of the tissue-specific proapoptotic BH3-only protein Nbk/Bik is a unifying feature of renal cell carcinoma.Cell Death Differ. 13: 619-627.
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