|Application ||WB, IHC-F|
|Calculated MW||18016 Da|
|Purification||Antisera to Bik were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG prepared by affinity chromatography.|
|Immunogen||15 amino acid peptide from near the amino terminus of human Bik.|
|Shelf Life||18 months from date of despatch.|
|Other Names||Bcl-2-interacting killer, Apoptosis inducer NBK, BIP1, BP4, BIK, NBK|
|Target/Specificity||ABD10936 detects an epitope within the N-terminal (NT) region of Bcl-2-interacting killer (Bik) also known as natural born killer (Nbk). Bik is a Bcl-2 homology domain 3 (BH3)-only protein of 18kD which requires the BAX gene for activation. Bik forms heterodimers with various antiapoptotic proteins such as Bcl-2 and Bcl-XL, inhibiting their antiapoptotic function. This can result in the release of Ca2+ and cytochrome C into the cytosol, leading to apoptosis. Loss of Bik is a common feature of clear-cell renal cell carcinoma (RCC), and is thought to act as a renal tissue-specific tumour suppressor gene.|
|Preservative & Stabilisers||0.02% Sodium Azide|
|Storage||Store at +4℃ or at -20 ℃.|
|Precautions||Anti-BIK (N-Terminal) Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
1. Mathai, J.P. et al. (2005) BH3-only BIK regulates BAX,BAK-dependent release of Ca2+ from endoplasmic reticulum stores and mitochondrial apoptosis during stress-induced cell death. J. Biol. Chem. 280: 23829-23836.2. Real, P.J. et al. (2006) Transcriptional activation of the proapoptotic bik gene by E2F proteins in cancer cells.FEBS Lett. 580: 5905-5909.3. Sturm, I. et al. (2006) Loss of the tissue-specific proapoptotic BH3-only protein Nbk/Bik is a unifying feature of renal cell carcinoma.Cell Death Differ. 13: 619-627.
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