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Anti-Slug Antibody

Rabbit Anti-Human Polyclonal Antibody

  • IF - Anti-Slug Antibody  ABD10942
    Immunofluorescence staining of human kidney with Rabbit anti Human Slug
  • WB - Anti-Slug Antibody  ABD10942
    Western blot analysis of whole cell lysate from HEK293 human epithelial kidney cells probed with Rabbit anti Human Slug at 0.5(A), and 1(B) and 2(C) ug/ml
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession O43623
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Polyclonal IgG
Calculated MW 29986 Da
Additional Information
Other Species M
Purification Antisera to human Slug were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG prepared by affinity chromatography.
Immunogen 13 amino acid peptide sequence near the centre of human Slug.
Shelf Life 18 months from date of despatch.
Gene ID 6591
Other Names Zinc finger protein SNAI2, Neural crest transcription factor Slug, Protein snail homolog 2, SNAI2, SLUG, SLUGH
Target/Specificity Rabbit anti-Slug antibody detects Slug, a member of the Snail family of zinc finger transcription factors, whose expression is induced by FGF, BMP, and TGF-ß. Slug is a 29kD transcriptional repressor, which functions by binding to E-box motifs and inhibiting gene expression by recruiting histone deacetylase-1 (HDAC-1).Slug controls the expression of a wide variety of genes including TP53, BRCA2, PUMandCDH1, and has important roles in apoptosis and epithelial-mesenchymal transition. It is also thought to function in neural crest development.Slug expression in breast, oesophageal, and colorectal carcinomas has been correlated with poor prognosis for survival. Rabbit anti-Slug antibody is not cross-reactive with Snail.
Preservative & Stabilisers 0.02% Sodium Azide
Storage Store at +4℃ or at -20 ℃.
PrecautionsAnti-Slug Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
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1. Tripathi, M.K. et al. (2005) Regulation of BRCA2 gene expression by the SLUG repressor protein in human breast cells.
J. Biol. Chem. 280: 17163-17171.

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Cat# ABD10942
(40 western blots)
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