|Application ||WB, IHC-F|
|Calculated MW||36949 Da|
|Purification||Antisera to human BMI-1 were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG prepared by affinity chromatography.|
|Immunogen||15 amino acid peptide sequence near the centre of human BMI-1.|
|Shelf Life||18 months from date of despatch.|
|Other Names||Polycomb complex protein BMI-1, Polycomb group RING finger protein 4, RING finger protein 51, BMI1, PCGF4, RNF51|
|Target/Specificity||Rabbit anti-BMI-1 antibody recognizes 37kDa polycomb complex protein BMI-1, involved in segment specification, cell growth and maintenance, transcriptional regulation and chromatin modification. BMI-1 is a proto-oncogene which cooperates with the myc oncogene to produce B-lymphomas. It is required in the regulation of normal adult and leukaemic stem cells, making BMI-1 a potential target for stem cell proliferation and anti-cancer therapies. BMI-1 represses transcription at the ink4 locus which contains tumour suppressors p16 and p19Arf.BMI-1 is a component of the Polycomb group (PcG) multiprotein PRC1 complex which ubiquitinates histone-2A at lys119, resulting in a heritable change in the ability of chromatin to express genes. BMI-1 is also required to stimulate the E3 ubiquitin ligase activity of RNF2/RING2 within the PRC1 complex.The Notch ligand DLL4 (delta-like protein 4) enhances T cell proliferation, by directly influencing BMI-1 transcription.|
|Preservative & Stabilisers||0.02% Sodium Azide|
|Storage||Store at +4℃ or at -20 ℃.|
|Precautions||Anti-BMI-1 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
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Provided below are standard protocols that you may find useful for product applications.
1. Raaphorst, F. et al. (2003) Self-renewal of hematopoietic and leukemic stem cells: a central role for the Polycomb-group gene Bmi-1. Trends Immunol. 10:522-4.2. Alkema, M. et al. (1993) Characterization and chromosomal localization of the human proto-oncogene BMI-1.Hum. Mol. Genet. 2:1597-1603.3. Lessard, J. et al. (2003) BMI-1 determines the proliferative capacity of normal and leukaemic stem cells. Nature 423:255-260.
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