|Application ||WB, IHC-P|
|Calculated MW||58043 Da|
|Purification||Antisera to human PAK2 were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG prepared by affinity chromatography.|
|Immunogen||14 amino acid peptide sequence near the carboxy terminus of human PAK2.|
|Shelf Life||18 months from date of despatch.|
|Other Names||Serine/threonine-protein kinase PAK 2, 18.104.22.168, Gamma-PAK, PAK65, S6/H4 kinase, p21-activated kinase 2, PAK-2, p58, PAK-2p27, p27, PAK-2p34, p34, C-t-PAK2, PAK2|
|Target/Specificity||ABD10945 recognises p21-activated kinase-2 (PAK2), a ubiquitously expressed, 64kDa, serine/threonine protease found at higher levels in skeletal muscle, ovary, thymus and spleen. p21-activated kinases (PAKs) bind activated Cdc42 and Rac via a Cdc42/Rac interactive binding (CRIB) domain. Group 1 PAK kinases (PAK1, 2 and 3) regulate the activity of an adjacent conserved autoinhibitory domain and are associated with cellular processes, including cytoskeleton rearrangement, apoptosis and MAP kinase signalling. Group 2 PAKs (PAK4, 5, and 6) lack the autoinhibitory domain and are not activated by Cdc42/Rac binding. PAK2 has a cytostatic effect on cells and is activated in response to cellular stresses such hyperosmolarity, serum starvation, contact inhibition and DNA damage from radiation and chemicals. Unlike other PAKs, PAK2 can be activated by proteolytic cleavage to generate a constitutively active fragment, PAK2p34, which induces a cell death response. This induction is thought to be regulated by Rho GTPase-activating protein 10 which inhibits the protein kinase activity of PAK2p34 and causes it to localise to the perinuclear region instead of the nucleus. PAK2 may also play a role in X-linked mental retardation.|
|Preservative & Stabilisers||0.02% Sodium Azide|
|Storage||Store at +4℃ or at -20 ℃.|
|Precautions||Anti-PAK2 (C-Terminal) Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
1. Roig, J. et al. (2001) Cytostatic p21 G protein-activated protein kinase gamma-PAK.Vitam Horm. 62:167-198.2. Rudel, T. et al. (1997) Membrane and morphological changes in apoptotic cells regulated by caspase-mediated activation of PAK2.Science. 276:1571-1574.3. Jaffer, Z. et al. (2002) p21-activated kinases: three more join the Pak.Int. J. Biochem. Cell. Biol. 34:713-717.
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