|Calculated MW||72928 Da|
|Purification||Antisera to rat Raf-1 (pSer 642) were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG prepared affinity chromatography.|
|Immunogen||Phosphopeptide corresponding to animo acid sequence surrounding phosphorylated Ser 642 of rat Raf-1.|
|Shelf Life||12 months from date of reconstitution.|
|Other Names||RAF proto-oncogene serine/threonine-protein kinase, 220.127.116.11, Proto-oncogene c-RAF, cRaf, Raf-1, Raf1, Raf|
|Target/Specificity||ABD10959 recognises Raf-1 phosphorylated at serine 642, a ubiquitously expressed 74 kDa member of the serine/threonine protein kinase family. Raf-1 is a MAP kinase kinase kinase and participates in the transduction of mitogenic signals from receptors on the cell membrane to the nucleus. Raf-1 is activated through phosphorylation by Ras and in turn phosphorylates MEK which activates ERK. ERK when activated regulates the expression of genes involved in cell cycle, differentiation, migration and apoptosis. As such Raf-1 is an important clinical target in cancer.Raf-1 activity can be downregulated by phosphorylation at serine residues 301 and 642.|
|Preservative & Stabilisers||0.09% Sodium Azide (NaN3); 0.01% Bovine Serum Albumin; 50% Glycerol|
|Storage||Store at +4℃ or at -20 ℃.|
|Precautions||Anti-RAF-1 (pSer642) Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
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