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>   home   >   Products   >   Primary Antibodies   >   Anti-RAF-1 (pSer642) Antibody    

Anti-RAF-1 (pSer642) Antibody

Rabbit Anti-Rat Polyclonal Antibody

     
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB
Primary Accession P11345
Reactivity Rat
Host Rabbit
Clonality Polyclonal
Isotype Polyclonal IgG
Calculated MW 72928 Da
Additional Information
Purification Antisera to rat Raf-1 (pSer 642) were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG prepared affinity chromatography.
Immunogen Phosphopeptide corresponding to animo acid sequence surrounding phosphorylated Ser 642 of rat Raf-1.
Shelf Life 12 months from date of reconstitution.
Gene ID 24703
Other Names RAF proto-oncogene serine/threonine-protein kinase, 2.7.11.1, Proto-oncogene c-RAF, cRaf, Raf-1, Raf1, Raf
Target/Specificity ABD10959 recognises Raf-1 phosphorylated at serine 642, a ubiquitously expressed 74 kDa member of the serine/threonine protein kinase family. Raf-1 is a MAP kinase kinase kinase and participates in the transduction of mitogenic signals from receptors on the cell membrane to the nucleus. Raf-1 is activated through phosphorylation by Ras and in turn phosphorylates MEK which activates ERK. ERK when activated regulates the expression of genes involved in cell cycle, differentiation, migration and apoptosis. As such Raf-1 is an important clinical target in cancer.Raf-1 activity can be downregulated by phosphorylation at serine residues 301 and 642.
Preservative & Stabilisers 0.09% Sodium Azide (NaN3); 0.01% Bovine Serum Albumin; 50% Glycerol
Storage Store at +4℃ or at -20 ℃.
PrecautionsAnti-RAF-1 (pSer642) Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Citations (0)

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References

1. Gollob, J. et al. (2006) Role of Raf kinase in cancer: therapeutic potential of targeting the Raf/MEK/ERK signal transduction pathway.
Semin. Oncol. 4:392-406. 2. Dougherty, M. et al. (2005) Regulation of Raf-1 by direct feedback phosphorylation.
Mol. Cell. 17:215-24.

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Cat# ABD10959
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