|Calculated MW||47399 Da|
|Purification||Antisera to synaptotagmin 1 (pSer309) were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG prepared by affinity chromatography.|
|Immunogen||Phosphopeptide corresponding to amino acid sequence surrounding phosphorylated serine 309 of synaptotagmin 1.|
|Shelf Life||12 months from date of reconstitution.|
|Other Names||Synaptotagmin-1, Synaptotagmin I, SytI, p65, Syt1|
|Target/Specificity||ABD10961 recognises synaptotagmin 1 phosphorylated at serine 309, a 60kDa member of the synaptotagmin family, expressed in membranes of synaptic vesicles in rostral, phylogenetically younger brain regions and in some endocrine tissues. Ca2+ is bound by synaptogamin whose C2 domains then bind phospholipids, trafficking vesicles to the synaptic membrane and exocytosis. This results in fast synchronous neurotransmitter release. Synaptotagmin also binds neurexins, syntaxin and AP2 independently of Ca2+ binding.Phosphorylation of synaptotagmin by protein kinases is thought to modulate its activity.|
|Preservative & Stabilisers||0.09% Sodium Azide (NaN3); 0.01% Bovine Serum Albumin; 50% Glycerol|
|Storage||Store at +4℃ or at -20 ℃.|
|Precautions||Anti-Synaptotagmin 1 (pSer309) Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
1. Xu, Jun. et al. (2007) Synaptotagmin-1, -2, and -9: Ca2+ Sensors for Fast Release that Specify Distinct Presynaptic Properties in Subsets of Neurons. Neuron. Volume 54:567-581. 2. Sullivan, J. (2007) Please Release Me.Neuron. Volume 54:493-494. 3. Hilfiker, S. et al. (1999) Regulation of synaptotagmin I phosphorylation by multiple protein kinases.J. Neurochem. 73:921-932.
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