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Anti-Synaptotagmin 1 (pSer309) Antibody

Rabbit Anti-Rat Polyclonal Antibody

  • WB - Anti-Synaptotagmin 1 (pSer309) Antibody  ABD10961
    Western blot of rat cortex lysate showing phosphospecific immunolabeling of the ~60 kDa and ~62 kDa synaptotagmin phosphorylated at Se369
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P21707
Reactivity Rat
Host Rabbit
Clonality Polyclonal
Isotype Polyclonal IgG
Calculated MW 47399 Da
Additional Information
Purification Antisera to synaptotagmin 1 (pSer309) were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG prepared by affinity chromatography.
Immunogen Phosphopeptide corresponding to amino acid sequence surrounding phosphorylated serine 309 of synaptotagmin 1.
Shelf Life 12 months from date of reconstitution.
Gene ID 25716
Other Names Synaptotagmin-1, Synaptotagmin I, SytI, p65, Syt1
Target/Specificity ABD10961 recognises synaptotagmin 1 phosphorylated at serine 309, a 60kDa member of the synaptotagmin family, expressed in membranes of synaptic vesicles in rostral, phylogenetically younger brain regions and in some endocrine tissues. Ca2+ is bound by synaptogamin whose C2 domains then bind phospholipids, trafficking vesicles to the synaptic membrane and exocytosis. This results in fast synchronous neurotransmitter release. Synaptotagmin also binds neurexins, syntaxin and AP2 independently of Ca2+ binding.Phosphorylation of synaptotagmin by protein kinases is thought to modulate its activity.
Preservative & Stabilisers 0.09% Sodium Azide (NaN3); 0.01% Bovine Serum Albumin; 50% Glycerol
Storage Store at +4℃ or at -20 ℃.
PrecautionsAnti-Synaptotagmin 1 (pSer309) Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Citations (0)

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1. Xu, Jun. et al. (2007) Synaptotagmin-1, -2, and -9: Ca2+ Sensors for Fast Release that Specify Distinct Presynaptic Properties in Subsets of Neurons.
Neuron. Volume 54:567-581. 2. Sullivan, J. (2007) Please Release Me.
Neuron. Volume 54:493-494. 3. Hilfiker, S. et al. (1999) Regulation of synaptotagmin I phosphorylation by multiple protein kinases.
J. Neurochem. 73:921-932.

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Cat# ABD10961
(40 western blots)
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