|Application ||WB, E|
|Calculated MW||154537 Da|
|Purification||Antisera to ASK1 (pSER83) were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by ion exchange chromatography.|
|Immunogen||KLH conjugated peptide corresponding to amino acids 76-87 of human ASK-1 protein.|
|Shelf Life||18 months from date of despatch.|
|Other Names||Mitogen-activated protein kinase kinase kinase 5, 188.8.131.52, Apoptosis signal-regulating kinase 1, ASK-1, MAPK/ERK kinase kinase 5, MEK kinase 5, MEKK 5, MAP3K5, ASK1, MAPKKK5, MEKK5|
|Target/Specificity||ABD10962 detects Apoptosis Signal-regulating Kinase 1 (ASK1) phosphorylated at serine83. ASK1 is a serine/threonine kinase also known as MAP kinase kinase kinase (MAPKKK), and plays an important role in the apoptotic response to various stimuli including oxidative stress, cytokines (e.g. TNF) and endoplasmic reticulum stress. Akt in conjunction to Hsp90 phosphorylates ASK1 at Ser83 to inhibit ASK1 induced apoptosis.|
|Preservative & Stabilisers||0.01% Sodium Azide (NaN3)|
|Storage||Store at +4℃ or at -20 ℃.|
|Precautions||Anti-Human ASK1 (pSer83) Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
1. Zhang, R. et al. (2005) Hsp90-Akt phosphorylates ASK1 and inhibits ASK1-mediated apoptosis.Oncogene. 24: 3954-3963.2. Hayakawa, T. et al. (2006) The ASK1-MAP kinase pathways in immune and stress responses.Microbes Infect. 8: 1098-1107.3. Takeda, K. et al. (2003) Roles of MAPKKK ASK1 in stress-induced cell death.Cell Struct. Funct. 28: 23-29.
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