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>   home   >   Products   >   Primary Antibodies   >   Anti-Yeast RAD9 Antibody    

Anti-Yeast RAD9 Antibody

Rabbit Anti-Yeast Polyclonal Antibody

Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P14737
Reactivity Yeast
Host Rabbit
Clonality Polyclonal
Isotype Polyclonal IgG
Calculated MW 148398 Da
Additional Information
Purification Antisera to yeast Rad9 were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
Immunogen Synthetic peptide corresponding to aa 1125-1139 of yeast Rad9 protein conjugated to Keyhole Limpet Haemocyanin (KLH).
Shelf Life 18 months from date of despatch.
Gene ID 851803
Other Names DNA repair protein RAD9, RAD9
Target/Specificity ABD10982 detects Saccharomyces cerevisiae Rad9, a protein involved in the DNA damage checkpoint. In S. cerevisiae this checkpoint is mainly controlled by the PI(3) kinase-like kinase (PIKK) Mec1, which is activated in response to DNA damage. Activated Mec1 hyperphosphorylates Rad9, which in turn triggers the binding of Rad53, the orthologue of mammalian Chk2. Activated Rad53 specifically targets substrates required for cell cycle arrest, gene expression and efficient DNA repair. Mutations affecting Rad9 impair checkpoint induced cell-cycle arrest and increase genomic instability. This antibody is pan reactive with both the phosphorylated and non-phosphorylated forms of this protein.
Preservative & Stabilisers 0.09% Sodium Azide (NaN3)
Storage Store at +4℃ or at -20 ℃.
PrecautionsAnti-Yeast RAD9 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Citations (0)

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1. Toh, G.W. and Lowndes, N.F. (2003) Role of the Saccharomyces cerevisiae Rad9 protein in sensing and responding to DNA damage.
Biochem. Soc. Trans. 31(Pt 1): 242-246. 2. Sweeney, F.D. et al. (2005) Saccharomyces cerevisiae Rad9 acts as a Mec1 adaptor to allow Rad53 activation.
Curr. Biol. 15: 1364-1375.

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Cat# ABD10982
(40 western blots)
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