|Calculated MW||28931 Da|
|Purification||Antisera to rat aquaporin 2 were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG was prepared by affinity chromatography.|
|Immunogen||Synthetic phosphopeptide corresponding to an amino acid sequence within Aquaporin 2, which includes phosphorylated Ser261.|
|Shelf Life||12 months from date of reconstitution.|
|Other Names||Aquaporin-2, AQP-2, ADH water channel, Aquaporin-CD, AQP-CD, Collecting duct water channel protein, WCH-CD, Water channel protein for renal collecting duct, Aqp2|
|Target/Specificity||ABD10984 specifically recognises the 29kDa integral membrane protein Aquaporin 2 (AQP2), when phosphorylated at Ser261. AQP2 provides the plasma membranes of the renal collecting duct with a high permeability channel to enable the movement of water across an osmotic gradient, under hormonal regulation by vasopressin. In addition to the Ser256 phosphorylation site, studies have identified a novel phosphorylated Ser261 site on AQP2, which decreases in expression, in response to vasopressin. Gene mutations in AQP2 result in hereditary nephrogenic diabetes insipidus in humans, and acquired diabetes insipidus can also occur through a decrease in the expression of the AQP2 gene, following lithium treatment. Expression of the AQP2 gene is increased during pregnancy and other conditions associated with water retention.|
|Preservative & Stabilisers||0.09% Sodium Azide (NaN3); 0.01% Bovine Serum Albumin; 50% Glycerol|
|Storage||Store at +4℃ or at -20 ℃.|
|Precautions||Anti-Rat Aquaporin 2 (pSer261) Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
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Provided below are standard protocols that you may find useful for product applications.
1. Hoffert, J.D. et al. (2007) Dynamics of aquaporin-2 serine-261 phosphorylation in response to short-term vasopressin treatment in collecting duct.Am. J. Physiol. Renal Physiol. 292: 691-700.1. Liu, H. and Wintour, E.M. (2005) Aquaporins in development – a review.Reprod. Biol. Endocrinol. 3: 18-27.
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