|Calculated MW||50198 Da|
|Purification||Antisera to MEK5 were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG was prepared by affinity chromatography.|
|Immunogen||Synthetic phosphopeptide corresponding to an amino acid sequence within MEK5 , which includes phoshorylated Ser311 and Thr315.|
|Shelf Life||12 months from date of reconstitution.|
|Other Names||Dual specificity mitogen-activated protein kinase kinase 5, MAP kinase kinase 5, MAPKK 5, 22.214.171.124, MAPK/ERK kinase 5, MEK 5, Map2k5, Mek5, Mkk5, Prkmk5|
|Target/Specificity||ABD10987 specifically recognises 49kDa MEK5, also known as MAP2K5, when phosphorylated at Ser311 and Thr315. MEK5 is a dual specificity serine/threonine protein kinase belonging to the MAP kinase kinase family, which acts as a key regulator in a mitogen-activated protein (MAP) kinase cascade, and hence growth factor-induced cellular stimulation. MEK5 exists in naturally occurring MEK5alpha and MEK5beta splice variants, the former of which is known to specifically activate/regulate MAPK7/ERK5/BMK1, whilst the latter may act as a suppressor of MAPK7 activation. Activation of MEK5 itself is regulated by upstream MAP3K3/MEKK3, and phosphorylation of MEK5 at both the Ser311 and Thr315 sites has been implicated in cell proliferation.|
|Preservative & Stabilisers||0.09% Sodium Azide (NaN3); 0.01% Bovine Serum Albumin; 50% Glycerol|
|Storage||Store at +4℃ or at -20 ℃.|
|Precautions||Anti-Rat MEK 5 (pSer311/Thr315) Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
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Provided below are standard protocols that you may find useful for product applications.
1. Cameron, S.J. et al. (2004) Differential role of MEK5alpha and MEK5beta in BMK1/ERK5 activation.J. Biol. Chem. 279: 1506-1512.
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