|Calculated MW||50198 Da|
|Purification||Antisera to MEK5 were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG was prepared by affinity chromatography.|
|Immunogen||Synthetic phosphopeptide corresponding to an amino acid sequence within MEK5 , which includes phoshorylated Ser311 and Thr315.|
|Shelf Life||12 months from date of reconstitution.|
|Other Names||Dual specificity mitogen-activated protein kinase kinase 5, MAP kinase kinase 5, MAPKK 5, 184.108.40.206, MAPK/ERK kinase 5, MEK 5, Map2k5, Mek5, Mkk5, Prkmk5|
|Target/Specificity||ABD10987 specifically recognises 49kDa MEK5, also known as MAP2K5, when phosphorylated at Ser311 and Thr315. MEK5 is a dual specificity serine/threonine protein kinase belonging to the MAP kinase kinase family, which acts as a key regulator in a mitogen-activated protein (MAP) kinase cascade, and hence growth factor-induced cellular stimulation. MEK5 exists in naturally occurring MEK5alpha and MEK5beta splice variants, the former of which is known to specifically activate/regulate MAPK7/ERK5/BMK1, whilst the latter may act as a suppressor of MAPK7 activation. Activation of MEK5 itself is regulated by upstream MAP3K3/MEKK3, and phosphorylation of MEK5 at both the Ser311 and Thr315 sites has been implicated in cell proliferation.|
|Preservative & Stabilisers||0.09% Sodium Azide (NaN3); 0.01% Bovine Serum Albumin; 50% Glycerol|
|Storage||Store at +4℃ or at -20 ℃.|
|Precautions||Anti-Rat MEK 5 (pSer311/Thr315) Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
firstname.lastname@example.org, and receive a free "I Love Antibodies" mug.
Provided below are standard protocols that you may find useful for product applications.
1. Cameron, S.J. et al. (2004) Differential role of MEK5alpha and MEK5beta in BMK1/ERK5 activation.J. Biol. Chem. 279: 1506-1512.
If you have used an Abgent product and would like to share how it has performed, please click on the "Submit Review" button and provide the requested information. Our staff will examine and post your review and contact you if needed.
If you have any additional inquiries please email technical services at email@example.com.