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>   home   >   Products   >   Primary Antibodies   >   Anti-Rat MEK 5 (pSer311/Thr315) Antibody    

Anti-Rat MEK 5 (pSer311/Thr315) Antibody

Rabbit Anti-Rat Polyclonal Antibody

  • WB - Anti-Rat MEK 5 (pSer311/Thr315) Antibody  ABD10987
    Western blot of rat testes lysate showing phospho-specific immunolabeling of the ~49 kDa MEK5 protein phosphorylated at Ser311 and Thr315
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q62862
Reactivity Rat
Host Rabbit
Clonality Polyclonal
Isotype Polyclonal IgG
Calculated MW 50198 Da
Additional Information
Purification Antisera to MEK5 were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG was prepared by affinity chromatography.
Immunogen Synthetic phosphopeptide corresponding to an amino acid sequence within MEK5 , which includes phoshorylated Ser311 and Thr315.
Shelf Life 12 months from date of reconstitution.
Gene ID 29568
Other Names Dual specificity mitogen-activated protein kinase kinase 5, MAP kinase kinase 5, MAPKK 5,, MAPK/ERK kinase 5, MEK 5, Map2k5, Mek5, Mkk5, Prkmk5
Target/Specificity ABD10987 specifically recognises 49kDa MEK5, also known as MAP2K5, when phosphorylated at Ser311 and Thr315. MEK5 is a dual specificity serine/threonine protein kinase belonging to the MAP kinase kinase family, which acts as a key regulator in a mitogen-activated protein (MAP) kinase cascade, and hence growth factor-induced cellular stimulation. MEK5 exists in naturally occurring MEK5alpha and MEK5beta splice variants, the former of which is known to specifically activate/regulate MAPK7/ERK5/BMK1, whilst the latter may act as a suppressor of MAPK7 activation. Activation of MEK5 itself is regulated by upstream MAP3K3/MEKK3, and phosphorylation of MEK5 at both the Ser311 and Thr315 sites has been implicated in cell proliferation.
Preservative & Stabilisers 0.09% Sodium Azide (NaN3); 0.01% Bovine Serum Albumin; 50% Glycerol
Storage Store at +4℃ or at -20 ℃.
PrecautionsAnti-Rat MEK 5 (pSer311/Thr315) Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Citations (0)

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1. Cameron, S.J. et al. (2004) Differential role of MEK5alpha and MEK5beta in BMK1/ERK5 activation.
J. Biol. Chem. 279: 1506-1512.

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Cat# ABD10987
(40 western blots)
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