|Calculated MW||166071 Da|
|Purification||Antisera to rat NMDA receptor NR2B subunit were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG was prepared by affinity chromatography.|
|Immunogen||Synthetic phosphopeptide corresponding to an amino acid sequence within the NR2B subunit of the rat NMDA receptor, which includes phoshorylated Ser1480.|
|Shelf Life||12 months from date of reconstitution.|
|Other Names||Glutamate receptor ionotropic, NMDA 2B, GluN2B, Glutamate [NMDA] receptor subunit epsilon-2, N-methyl D-aspartate receptor subtype 2B, NMDAR2B, NR2B, Grin2b|
|Target/Specificity||ABD10988 specifically recognises the 180kDa NR2B subunit of the NMDA (N-methyl-D-aspartate) receptor, when phosphorylated at Ser1480.Receptors for NMDA belong to a group of ionotropic glutamate receptors which play a key role in the mediation of glutamate neurotransmission within the mammalian central nervous system (CNS), including involvement in memory and learning processes. Several antagonists and agonists of NMDA receptors (NMDAR) have been identified, including the glutamate analogue homoquinolinic acid, which displays a higher affinity for NR2B-containing NMDAR. Properties of NMDAR include modulation by glycine, inhibition by Zn2+, voltage-dependent Mg2+ blockade and high Ca2+permeability. Studies have shown that the phosphorylation of the Ser1480 site within the C-terminal PDZ ligand of the NR2B subunit of NMDAR, by casein kinase II (CK2), results in a decrease in neuron NR2B surface expression, see Chung, H.J. et al. for details.The involvement of NMDAR in the CNS has become a focus area for neurodegenerative diseases such as Alzheimer’s disease (Popke, E.J.) and also epilepsy and ischemic neuronal cell death.|
|Preservative & Stabilisers||0.09% Sodium Azide (NaN3); 0.01% Bovine Serum Albumin; 50% Glycerol|
|Storage||Store at +4℃ or at -20 ℃.|
|Precautions||Anti-Rat NMDA Receptor NR2B (pSer1480) Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
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Provided below are standard protocols that you may find useful for product applications.
1. Chung, H.J. et al. (2004) Regulation of the NMDA receptor complex and trafficking by activity-dependent phosphorylation of the NR2B subunit PDZ ligand. J. Neurosci. 24: 10248-10259.2. Popke, E.J. (2003) From anticholinesterase toxicity to Alzheimer’s disease: Important interactions of cholinergic and NMDA receptor systems. Toxological Sciences 72: 185-187.
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