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Anti-Human Parkin (pSer101) Antibody

Rabbit Anti-Human Polyclonal Antibody

  • WB - Anti-Human Parkin (pSer101) Antibody  ABD10989
    Western blot of HEK293 cells transfected with Parkin wt(phospho) and Parkin ser101 mutant (non-phospho) showing the phospho-specific immunolabeling of the ~52 kDa Parkin protein in the wild type and not in the mutant
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession O60260
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Polyclonal IgG
Calculated MW 51641 Da
Additional Information
Purification Antisera to human parkin were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG was prepared by affinity chromatography.
Immunogen Synthetic phosphopeptide corresponding to an amino acid sequence within parkin, which includes phosphorylated Ser101.
Shelf Life 12 months from date of reconstitution.
Gene ID 5071
Other Names E3 ubiquitin-protein ligase parkin, 6.3.2.-, Parkinson juvenile disease protein 2, Parkinson disease protein 2, PARK2, PRKN
Target/Specificity ABD10989 specifically recognises the 52kDa parkin protein, when phosphorylated at Ser101.Parkin is an E3 ubiquitin ligase highly expressed in the brain, which functions in the ubiquitin-proteasome system, together with the E2 ubiquitin conjugating co-enzyme UbcH7. Mutations in the parkin gene are associated with the hereditary neurodegenerative disorder known as autosomal recessive early onset Parkinson disease 2 (PARK2)/autosomal recessive juvenile Parkinson disease (PDJ), characterized by progressive dopaminergic neural cell death. Studies have identified the presence of at least five phosphorylation sites in parkin including Ser101, shown to be phosphorylated by Casein kinase-1 (CK-1), and suggest that phosphorylation of parkin may act to regulate its ubiquitin ligase activity.
Preservative & Stabilisers 0.09% Sodium Azide (NaN3); 0.01% Bovine Serum Albumin; 50% Glycerol
Storage Store at +4℃ or at -20 ℃.
PrecautionsAnti-Human Parkin (pSer101) Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
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Cat# ABD10989
(40 western blots)
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