|Calculated MW||55621 Da|
|Purification||Antisera to rat tryptophan hydroxylase 2 were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG was prepared by affinity chromatography.|
|Immunogen||Synthetic phospeptide corresponding to an amino acid sequence within TPH2, which includes phosphorylated Ser19.|
|Shelf Life||12 months from date of reconstitution.|
|Other Names||Tryptophan 5-hydroxylase 2, 18.104.22.168, Neuronal tryptophan hydroxylase, Tryptophan 5-monooxygenase 2, Tph2, Ntph|
|Target/Specificity||ABD10991 specifically recognises the 55kDa Tryptophan Hydroxylase 2 (TPH2) protein, when phosphorylated at Ser19.Tryptophan Hydroxylase (TPH) is a rate-limiting enzyme which catalyses the 5-hydroxylation of tryptophan, to form the amino acid 5-hydroxytryptophan (5-HTP), in the first stage of the biosynthesis of the neurotransmitter serotonin (5-hydroxytryptamine/5HT). Mammalian TPH is encoded by two genes: TPH1 expressed in peripheral tissues e.g. skin and gut, and TPH2 primarily expressed in the brain. Studies have demonstrated the regulation/enhancement of TPH activity through phosphorylation by cAMP-dependent protein kinase (PKA) and Ca2+/calmodulin kinase II (CaMKII), the latter of which phosphorylates a Ser19 site within the regulatory domain of TPH2.|
|Preservative & Stabilisers||0.09% Sodium Azide (NaN3); 0.01% Bovine Serum Albumin; 50% Glycerol|
|Storage||Store at +4℃ or at -20 ℃.|
|Precautions||Anti-Rat Tryptophan Hydroxylase 2 (pSer19) Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
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Provided below are standard protocols that you may find useful for product applications.
1. McKinney, J. et al. (2005) Different properties of the central and peripheral forms of human tryptophan hydroxylase.J. Neurochem. 92: 311-320
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