|Application ||WB, E|
|Calculated MW||54558 Da|
|Purification||Antisera to Mdm2 were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG prepared by affinity chromatography.|
|Immunogen||Synthetic peptide corresponding to aa 177-195 of mouse Mdm2.|
|Shelf Life||18 months from date of despatch.|
|Other Names||E3 ubiquitin-protein ligase Mdm2, 6.3.2.-, Double minute 2 protein, Oncoprotein Mdm2, p53-binding protein Mdm2, Mdm2|
|Target/Specificity||ABD10999 detects murine Mdm2, a nuclear phosphoprotein that controls intracellular levels of the tumour suppressor protein p53 by ubiquitination and subsequent proteasomal degradation. Mdm2 also interacts with the retinoblastoma (RB) protein and E2F-1, to promote G1-S phase transition in the cell cycle. Mdm2 activity is controlled by self-ubiquitination; however activation of the PI3K/PKB pathway leads to phosphorylation of Mdm2 at Ser185, which stabilises the protein.|
|Preservative & Stabilisers||0.09% Sodium Azide (NaN3)|
|Storage||Store at +4℃ or at -20 ℃.|
|Precautions||Anti-Mouse MDM2 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
firstname.lastname@example.org, and receive a free "I Love Antibodies" mug.
Provided below are standard protocols that you may find useful for product applications.
1. Sdek, P. et al. (2004) The Central Acidic Domain of MDM2 Is Critical in Inhibition of Retinoblastoma-mediated Suppression of E2F and Cell Growth. J. Biol. Chem. 279: 53317 – 53322. 2. Feng J. et al. (2004) Stabilization of Mdm2 via decreased ubiquitination is mediated by protein kinase B/Akt-dependent phosphorylation. J. Biol. Chem. 279(34):35510-7.
If you have used an Abgent product and would like to share how it has performed, please click on the "Submit Review" button and provide the requested information. Our staff will examine and post your review and contact you if needed.
If you have any additional inquiries please email technical services at email@example.com.