|Application ||WB, E|
|Calculated MW||54558 Da|
|Purification||Antisera to Mdm2 were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG prepared by affinity chromatography.|
|Immunogen||Synthetic peptide corresponding to aa 177-195 of mouse Mdm2.|
|Shelf Life||18 months from date of despatch.|
|Other Names||E3 ubiquitin-protein ligase Mdm2, 6.3.2.-, Double minute 2 protein, Oncoprotein Mdm2, p53-binding protein Mdm2, Mdm2|
|Target/Specificity||ABD10999 detects murine Mdm2, a nuclear phosphoprotein that controls intracellular levels of the tumour suppressor protein p53 by ubiquitination and subsequent proteasomal degradation. Mdm2 also interacts with the retinoblastoma (RB) protein and E2F-1, to promote G1-S phase transition in the cell cycle. Mdm2 activity is controlled by self-ubiquitination; however activation of the PI3K/PKB pathway leads to phosphorylation of Mdm2 at Ser185, which stabilises the protein.|
|Preservative & Stabilisers||0.09% Sodium Azide (NaN3)|
|Storage||Store at +4℃ or at -20 ℃.|
|Precautions||Anti-Mouse MDM2 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
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Provided below are standard protocols that you may find useful for product applications.
1. Sdek, P. et al. (2004) The Central Acidic Domain of MDM2 Is Critical in Inhibition of Retinoblastoma-mediated Suppression of E2F and Cell Growth. J. Biol. Chem. 279: 53317 – 53322. 2. Feng J. et al. (2004) Stabilization of Mdm2 via decreased ubiquitination is mediated by protein kinase B/Akt-dependent phosphorylation. J. Biol. Chem. 279(34):35510-7.
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