|Application ||WB, E|
|Calculated MW||19827 Da|
|Purification||Antisera to human myosin regulatory light chain were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.|
|Immunogen||Synthetic peptide corresponding to amino acids 12-27 of human myosin regulatory light chain.|
|Shelf Life||18 months from date of despatch.|
|Other Names||Myosin regulatory light polypeptide 9, 20 kDa myosin light chain, LC20, MLC-2C, Myosin RLC, Myosin regulatory light chain 2, smooth muscle isoform, Myosin regulatory light chain 9, Myosin regulatory light chain MRLC1, MYL9, MLC2, MRLC1, MYRL2|
|Target/Specificity||ABD11002 detects human myosin regulatory light chain (MRLC), also known as MYL9 and LC20. Myosin is a hexamer composed of two high molecular weight heavy chains and four light chain subunits, which include two alkali light chains and two MRLCs. MRLC functions to stabilize the myosin heavy chain, and may also modulate the availability of cross bridges to bind to actin. Phosphorylation of the MRLC by myosin light chain kinase (MLCK) is necessary for smooth muscle contraction via activating myosin ATPase.This antibody does not detect the sarcomeric/cardiac myosin regulatory light chain isoform.|
|Preservative & Stabilisers||0.01% Sodium Azide (NaN3)|
|Storage||Store at +4℃ or at -20 ℃.|
|Precautions||Anti-Human Myosin Regulatory Light Chain Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
1. Nobe, H. et al. (2003) Rho kinase mediates serum-induced contraction in fibroblast fibers independent of myosin LC20 phosphorylation. Am. J. Physiol. Cell. Physiol. 284: C599-606.
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