|Application ||WB, E|
|Calculated MW||82705 Da|
|Purification||Antiserum to human Ku80 was raised by repeated immunisation of goats with highly purified antigen. Purified IgG was prepared by affinity chromatography.|
|Immunogen||Peptide sequence C-SLAKKDEKTDTLED from the internal region of Ku80 (NP_066964.1).|
|Shelf Life||18 months from date of despatch.|
|Other Names||X-ray repair cross-complementing protein 5, 3.6.4.-, 86 kDa subunit of Ku antigen, ATP-dependent DNA helicase 2 subunit 2, ATP-dependent DNA helicase II 80 kDa subunit, CTC box-binding factor 85 kDa subunit, CTC85, CTCBF, DNA repair protein XRCC5, Ku80, Ku86, Lupus Ku autoantigen protein p86, Nuclear factor IV, Thyroid-lupus autoantigen, TLAA, X-ray repair complementing defective repair in Chinese hamster cells 5 (double-strand-break rejoining), XRCC5, G22P2|
|Target/Specificity||ABD11024 specifically recognises the 80kDa subunit of human Ku protein, an evolutionarily conserved nuclear ATP-dependent DNA helicase, involved in a major proportion of DNA repair and in V(D)J recombination.The Ku protein, originally described as an autoantigen, exists as a tightly associated heterodimer consisting of a 70kDa (Ku70) and 80kDa (Ku80) subunit which binds to DNA double-strand break ends (DSB). DNA bound Ku recruits the large catalytic subunit DNA-PKcs to form the DNA-dependent protein kinase complex DNA-PK, facilitating DNA repair by the non-homologous end-joining (NHEJ) pathway.|
|Preservative & Stabilisers||0.02% Sodium Azide (NaN3); 0.5% Bovine Serum Albumin;|
|Storage||Store at +4℃ or at -20 ℃.|
|Precautions||Anti-Human Ku80 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
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Provided below are standard protocols that you may find useful for product applications.
1. Koike, M. (2002) Dimerization, translocation and localization of Ku70 and Ku80 proteins. J. Radiat. Res. 43: 223-236. 2. Dedieu, S. et al. (2010) The cytoprotective drug amifostine modifies both expression and activity of the pro-angiogenic factor VEGF-A.BMC Med. 8:19. 3. Slupianek, A. et al. (2011) BCR/ABL Stimulates WRN to Promote Survival and Genomic Instability.Cancer Res. 71: 842-51.
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