|Application ||WB, IHC-P, E|
|Calculated MW||28769 Da|
|Purification||Antiserum to human PCNA was raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.|
|Immunogen||Synthetic peptide corresponding an internal region of human PCNA protein.|
|Shelf Life||18 months from date of despatch.|
|Other Names||Proliferating cell nuclear antigen, PCNA, Cyclin, PCNA|
|Target/Specificity||Rabbit anti-Human PCNA antibody recognizes Human Proliferating Cell Nuclear Antigen (PCNA), also known as cyclin. PCNA is a 261 amino acid ~36kDa nuclear protein vital for cellular DNA synthesis, playing a key role in the DNA damage response where it acts as a loading platform to recruit additional damage repair proteins to lesions following DNA damage and promote repair (Burkovicset al.2009).PCNA is a useful marker for DNA synthesis and is highly conserved among most species. Rabbit anti-human PCNA antibody is expected to have wide species cross reactivity. PCNA expression in human dermis is negatively correlated to age as demonstrated by immunohustochemistry on paraffin sections using rabbit anti-human PCNA antibody (Guninet al.2014).|
|Preservative & Stabilisers||0.09% Sodium Azide (NaN3)|
|Storage||Store at +4℃ or at -20 ℃.|
|Precautions||Anti-PCNA Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
1. Gunin, A.G. et al. (2010) Age-Related Changes in Proliferation, the Numbers of Mast Cells, Eosinophils, and cd45-Positive Cells in Human DermisJ Gerontol A Biol Sci Med Sci. 66: 385-92. 2. Kim, J.Y. et al. (2012) Localization of osteopontin and osterix in periodontal tissue during orthodontic tooth movement in rats.Angle Orthod. 82: 107-14. 3. Petrov. V.V. et al. (2013) Age-related changes in mast cells and eosinophils of human dermisOntogenez. 44: 179-85. 4. Gunin, A.G. et al. (2014) Age-related changes in angiogenesis in human dermis.Exp Gerontol. 55C: 143-51.
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